Overview

  • Product name

    Anti-JNK1+JNK2 (phospho T183 + Y185) antibody
    See all JNK1+JNK2 primary antibodies
  • Description

    Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185)
  • Host species

    Rabbit
  • Specificity

    The region of JNK1 and JNK2 surrounding T183 + Y185 has a high degree of similarity to the corresponding regions in JNK3 and thus may cross react with this protein if phosphorylated on the corresponding residues.
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from around the phosphorylation sites of threonine 183/tyrosine 185 (

    M-M-T(p)-P-Y(p)- V - V

    ) of Human JNK1+JNK2 (NP_002741.1, NP_001128516.1, NP_002744.1)

  • Positive control

    • HeLa cells, C6 cells treated with anisomycin IHC-P: FFPE mouse brain tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab131499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 46, 54 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.
  • Information by UniProt
  • Database links

  • Alternative names

    • c jun N terminal kinase 2 antibody
    • c-Jun N-terminal kinase 1 antibody
    • cJun N terminal kinase 1 antibody
    • JNK 1 antibody
    • JNK 2 antibody
    • JNK-46 antibody
    • JNK2ALPHA antibody
    • JNK2BETA antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAPK 8 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • PRKM 8 antibody
    • PRKM 9 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • All lanes : Anti-JNK1+JNK2 (phospho T183 + Y185) antibody (ab131499) at 1/500 dilution

    Lane 1 : C6 cells treated with anisomycin
    Lane 2 : Untreated C6 cells

    Predicted band size: 46, 54 kDa



    Secondary antibody - anti-rabbit HRP (ab6721)

  • IHC image of JNK1 + JNK2 (phospho T183 + Y185) staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab131499, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131499 at 1/100 dilution.

References

This product has been referenced in:

  • Zhang BF  et al. KDM3A inhibition attenuates high concentration insulin-induced vascular smooth muscle cell injury by suppressing MAPK/NF-?B pathways. Int J Mol Med 41:1265-1274 (2018). Read more (PubMed: 29286083) »
  • Li XQ  et al. Liquiritin suppresses UVB-induced skin injury through prevention of inflammation, oxidative stress and apoptosis through the TLR4/MyD88/NF-?B and MAPK/caspase signaling pathways. Int J Mol Med 42:1445-1459 (2018). Read more (PubMed: 29901082) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116 colon cancer cell line)
Loading amount
30 µg
Specification
HCT116 colon cancer cell line
Treatment
DMSO control and 10 µM Irinotecan for 16 hrs
Gel Running Conditions
Reduced Denaturing (13%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Christian Marx

Verified customer

Submitted Feb 22 2013

Answer

Gracias por ponerte en contacto con nosotros y alertarnos del problema experimentado con nuestro producto. Cualquier comentario o queja procedente de nuestros clientes es muy importante para mejorar y monitorizar la calidad de nuestros productos. Para ello nos gustaría recoger más datos del protocolo seguido con el anticuerpo.

Te envío dos cuestionarios (para las dos aplicaciones para las que se ha validado este anticuerpo) que contiene algunas preguntas, y que no se tarda mucho en rellenar, y sin embargo a nosotros nos ayuda a llevar el registro de calidad de los productos y a sugeriros posibles mejoras en caso de encontrarlas. También te agradecería que añadieras alguna imagen que ilustre el problema sufrido.

Nuestra garantía Abpromise garantiza que los productos funcionan tal y como se especifica en su ficha técnica, y en caso contrario se os manda un anticuerpo de remplazo, se os reembolsa el dinero, o se emite un vale sin fecha de caducidad para futuras compras con nosotros.

Quedo entonces a la espera de tu respuesta. Por favor no dudes en contactarnos para cualquier otra duda o sugerencia.

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