Anti-JNK1 + JNK2 (phospho T183 + Y185) antibody (ab131499)
Key features and details
- Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185)
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-JNK1 + JNK2 (phospho T183 + Y185) antibody
See all JNK1 + JNK2 primary antibodies -
Description
Rabbit polyclonal to JNK1+JNK2 (phospho T183 + Y185) -
Host species
Rabbit -
Specificity
The region of JNK1 and JNK2 surrounding T183 + Y185 has a high degree of similarity to the corresponding regions in JNK3 and thus may cross react with this protein if phosphorylated on the corresponding residues. -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human JNK1 + JNK2 (phospho T183 + Y185). Synthetic peptide from around the phosphorylation sites of threonine 183/tyrosine 185 (
M-M-T(p)-P-Y(p)- V - V
) of Human JNK1 + JNK2 (NP_002741.1, NP_001128516.1, NP_002744.1) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 46, 54 kDa.
|
ICC/IF |
1/100 - 1/200.
|
Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 46, 54 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. - Information by UniProt
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Database links
- Entrez Gene: 5599 Human
- Entrez Gene: 5601 Human
- Entrez Gene: 26419 Mouse
- Entrez Gene: 26420 Mouse
- Entrez Gene: 116554 Rat
- Entrez Gene: 50658 Rat
- Omim: 601158 Human
- Omim: 602896 Human
see all -
Alternative names
- c jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 1 antibody
- cJun N terminal kinase 1 antibody
see all
Images
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All lanes : Anti-JNK1 + JNK2 (phospho T183 + Y185) antibody (ab131499) at 1/500 dilution
Lane 1 : C6 cells treated with anisomycin
Lane 2 : Untreated C6 cells
Predicted band size: 46, 54 kDaSecondary antibody - anti-rabbit HRP (ab6721)
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Immunofluorescence analysis of methanol fixed HeLa cells labelled with ab131499 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab131499 has been referenced in 23 publications.
- Chang HX & Zhao YF Protocatechuic acid as an inhibitor of the JNK/CXCL1/CXCR2 pathway relieves neuropathic pain in CCI rats. Bosn J Basic Med Sci 22:217-228 (2022). PubMed: 34813418
- Zhang LQ et al. DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation. J Neuroinflammation 19:129 (2022). PubMed: 35658977
- Liu Y et al. Deficiency in Inactive Rhomboid Protein2 (iRhom2) Alleviates Alcoholic Liver Fibrosis by Suppressing Inflammation and Oxidative Stress. Int J Mol Sci 23:N/A (2022). PubMed: 35887045
- Zheng M et al. Silencing ferritin alleviates atherosclerosis in mice via regulating the expression levels of matrix metalloproteinases and interleukins. Acta Biochim Pol 68:705-710 (2021). PubMed: 34730924
- Okada T et al. SPARC Aggravates Blood-Brain Barrier Disruption via Integrin αVβ3/MAPKs/MMP-9 Signaling Pathway after Subarachnoid Hemorrhage. Oxid Med Cell Longev 2021:9739977 (2021). PubMed: 34804372