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Signal Transduction Protein Phosphorylation Ser / Thr Kinases MAPK Pathway
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RecombinantRabMAb

Recombinant Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (7) Submit a question References (85)

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Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
  • Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16797-211] to JNK1+JNK2+JNK3
  • Suitable for: Flow Cyt, WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Chicken, Cow, Dog, Human, Zebrafish, African green monkey, Xenopus tropicalis, Recombinant fragment

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Overview

  • Product name

    Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]
    See all JNK1+JNK2+JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR16797-211] to JNK1+JNK2+JNK3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    Zebrafish
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human JNK1, JNK2 and JNK3 full length recombinant proteins; K562, HeLa, Jurkat, Neuro-2a, UMNSAH/DF-1, MDCK, MDBK and COS-1 whole cell lysates; Zebrafish and X. tropicalis lysates. Mouse brain, Rat brain, Rat heart, RAW 264.7, PC-12 and NIH/3T3 lysates. ICC/IF: HeLa cells. IP: Jurkat whole cell extract. Flow Cyt: HeLa cells
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR16797-211
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • MAPK pathway
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] - BSA and Azide free (ab225572)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IP
Human
WB
Mouse
Rat
Human
Zebrafish
Recombinant fragment
All applications
Monkey
Application Abreviews Notes
Flow Cyt
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB (4)
1/1000. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 48 kDa).
IP
1/50.
ICC/IF (3)
1/250.
Notes
Flow Cyt
1/180.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB
1/1000. Detects a band of approximately 54, 46 kDa (predicted molecular weight: 48 kDa).
IP
1/50.
ICC/IF
1/250.

Target

  • Function

    Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P45983 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5599 Human
    • Entrez Gene: 5601 Human
    • Entrez Gene: 5602 Human
    • Entrez Gene: 26414 Mouse
    • Entrez Gene: 26419 Mouse
    • Entrez Gene: 26420 Mouse
    • Entrez Gene: 116554 Rat
    • Entrez Gene: 25272 Rat
    • Entrez Gene: 50658 Rat
    • Entrez Gene: 65236 Zebrafish
    • Omim: 601158 Human
    • Omim: 602896 Human
    • Omim: 602897 Human
    • SwissProt: P45983 Human
    • SwissProt: P45984 Human
    • SwissProt: P53779 Human
    • SwissProt: Q61831 Mouse
    • SwissProt: Q91Y86 Mouse
    • SwissProt: Q9WTU6 Mouse
    • SwissProt: P49185 Rat
    • SwissProt: P49186 Rat
    • SwissProt: P49187 Rat
    • SwissProt: Q9DGD9 Zebrafish
    • Unigene: 138211 Human
    • Unigene: 522924 Human
    • Unigene: 21495 Mouse
    • Unigene: 4090 Rat
    • Unigene: 82505 Zebrafish
    see all
  • Alternative names

    • C Jun kinase 2 antibody
    • c Jun N terminal kinase 1 antibody
    • c Jun N terminal kinase 2 antibody
    • c Jun N terminal kinase 3 antibody
    • c-Jun N-terminal kinase 1 antibody
    • JNK 46 antibody
    • JNK 55 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1 antibody
    • JNK1+2+3 antibody
    • JNK1/2/3 antibody
    • JNK1A2 antibody
    • JNK2 antibody
    • JNK21B1/2 antibody
    • JNK2A antibody
    • JNK2ALPHA antibody
    • JNK2B antibody
    • JNK2BETA antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • Jun kinase antibody
    • JUN N terminal kinase antibody
    • MAP kinase 10 antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • MAPK 8 antibody
    • MAPK 9 antibody
    • MAPK10 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 10 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p493F12 antibody
    • p54a antibody
    • p54aSAPK antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • PRKM8 antibody
    • PRKM9 antibody
    • SAPK antibody
    • SAPK(beta) antibody
    • SAPK1 antibody
    • SAPK1a antibody
    • SAPK1b antibody
    • SAPK1c antibody
    • Stress activated protein kinase 1 antibody
    • Stress activated protein kinase 1a antibody
    • Stress activated protein kinase 1b antibody
    • Stress activated protein kinase 1c antibody
    • Stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK1 antibody
    • Stress activated protein kinase JNK2 antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase 1c antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with ab179461 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing both cytoplasmic and nuclear staining on HeLa cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: - ab179461 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    All lanes : Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
    Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

     

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

  • Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Immunocytochemistry/ Immunofluorescence - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

    Formaldehyde-fixed, NP40 permeabilized Mouse Vascular smooth muscle cells stained for JNK1+JNK2+JNK3 (Green) using ab179461 at 1/200 dilution followed by a Donkey anti-rabbit Alex Fluor® 488 antibody at 1/500 dilution. The nuclear counterstain was DAPI (Blue). 

  • Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Flow Cytometry - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling JNK1+JNK2+JNK3 with purified ab179461 at 1/180 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    All lanes : Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

    Lane 1 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates
    Lane 2 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
    Lane 3 : MDCK (Canine kidney cell line) whole cell lysates
    Lane 4 : MDBK (Bovine kidney cell line) whole cell lysates
    Lane 5 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

  • Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Immunoprecipitation - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

    JNK1+JNK2+JNK3 were immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell extract with ab179461 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179461 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Jurkat whole cell extract. Lane 2: PBS instead of Jurkat whole cell extract.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

     

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    All lanes : Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461) at 1/20000 dilution

    Lane 1 : Human JNK3 full length recombinant protein containing a proprietary tag.
    Lane 2 : Human JNK2 full length recombinant protein containing a proprietary tag.
    Lane 3 : Human JNK1 full length recombinant protein containing a His tag.

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Additional bands at: 48 kDa (possible tagged protein), 71 kDa (possible tagged protein), 71 kDa (possible tagged protein)



    Human JNK1, JNK2 and JNK3 full length recombinant proteins are from commercial sources. JNK1 and JNK2 have a proprietary tag, JNK3 has a His tag.

     

    Blocking/dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    All lanes : Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461) at 1/1000 dilution

    Lane 1 : Zebrafish lysate
    Lane 2 : X. tropicalis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    Zebrafish has only one JNK isoform, JNK1 with a MW of 44kDa. So there is only one band in Zebrafish.

  • Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Western blot - Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    All lanes : Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : Rat heart lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) lysate
    Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 46,54 kDa why is the actual band size different from the predicted?



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    JNKs originate from three genes that yield ten isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1 and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2 and JNK3β2, which represent the p54 isoforms.

  • Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)
    Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211] (ab179461)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (85)

    Publishing research using ab179461? Please let us know so that we can cite the reference in this datasheet.

    ab179461 has been referenced in 85 publications.

    • Jiang L  et al. Inhibition of microRNA-103 attenuates inflammation and endoplasmic reticulum stress in atherosclerosis through disrupting the PTEN-mediated MAPK signaling. J Cell Physiol 235:380-393 (2020). PubMed: 31232476
    • Hao Q  et al. Sulforaphane suppresses carcinogenesis of colorectal cancer through the ERK/Nrf2-UDP glucuronosyltransferase 1A metabolic axis activation. Oncol Rep 43:1067-1080 (2020). PubMed: 32323779
    • Fan H  et al. MsrA Suppresses Inflammatory Activation of Microglia and Oxidative Stress to Prevent Demyelination via Inhibition of the NOX2-MAPKs/NF-?B Signaling Pathway. Drug Des Devel Ther 14:1377-1389 (2020). PubMed: 32308370
    • Zhang Y  et al. EphB4/ TNFR2/ERK/MAPK signaling pathway comprises a signaling axis to mediate the positive effect of TNF-a on osteogenic differentiation. BMC Mol Cell Biol 21:29 (2020). PubMed: 32299362
    • Jia T  et al. MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR. Biosci Rep 40:N/A (2020). PubMed: 32202300
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-7 of 7 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Equus Cell (Tenocytes)
    Permeabilization
    Yes - 0.1% triton-X in PBS
    Specification
    Tenocytes
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 09 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Mouse Vascular smooth muscle cells)
    Permeabilization
    Yes - NP40
    Specification
    Mouse Vascular smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 03 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Permeabilization
    Yes - NP40
    Specification
    U2OS
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 21 2019

    Western blot abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Mouse Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    25 µg
    Specification
    Mouse Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 09 2019

    Western blot abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Vascular smooth muscle cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 29 2019

    Western blot abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (Neruo cell)
    Gel Running Conditions
    Reduced Denaturing (12 %)
    Loading amount
    20 µg
    Specification
    Neruo cell
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Miss. Ji Yeon Lee

    Verified customer

    Submitted Jan 29 2019

    Western blot abreview for Anti-JNK1+JNK2+JNK3 antibody [EPR16797-211]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human head and neck cell lines)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    human head and neck cell lines
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 09 2015

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