Recombinant
RabMAb

Recombinant Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] (Alexa Fluor® 488) (ab237117)

Overview

  • Product name

    Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] (Alexa Fluor® 488)
    See all JNK1+JNK2+JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18841-95] to JNK1+JNK2+JNK3 (Alexa Fluor® 488)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human JNK1+JNK2+JNK3 aa 150-400. The exact sequence is proprietary. SwissProt ID: P45984 = JNK2 and P53779 = JNK3.
    Database link: P45983

  • Positive control

    • ICC/IF: HeLa cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab237117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

This product gave a positive signal in HeLa cells fixed with 100% methanol (5 min)

Target

  • Function

    Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C Jun kinase 2 antibody
    • c Jun N terminal kinase 1 antibody
    • c Jun N terminal kinase 2 antibody
    • c Jun N terminal kinase 3 antibody
    • c-Jun N-terminal kinase 1 antibody
    • JNK 46 antibody
    • JNK 55 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1 antibody
    • JNK1+2+3 antibody
    • JNK1/2/3 antibody
    • JNK1A2 antibody
    • JNK2 antibody
    • JNK21B1/2 antibody
    • JNK2A antibody
    • JNK2ALPHA antibody
    • JNK2B antibody
    • JNK2BETA antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • Jun kinase antibody
    • JUN N terminal kinase antibody
    • MAP kinase 10 antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • MAPK 8 antibody
    • MAPK 9 antibody
    • MAPK10 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 10 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p493F12 antibody
    • p54a antibody
    • p54aSAPK antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • PRKM8 antibody
    • PRKM9 antibody
    • SAPK antibody
    • SAPK(beta) antibody
    • SAPK1 antibody
    • SAPK1a antibody
    • SAPK1b antibody
    • SAPK1c antibody
    • Stress activated protein kinase 1 antibody
    • Stress activated protein kinase 1a antibody
    • Stress activated protein kinase 1b antibody
    • Stress activated protein kinase 1c antibody
    • Stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK1 antibody
    • Stress activated protein kinase JNK2 antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase 1c antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • ab237117 staining JNK1+JNK2+JNK3 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab237117 at 1/1000 dilution (shown in green) and ab202272, Rabbit monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab237117 has not yet been referenced specifically in any publications.

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