Recombinant
RabMAb

Recombinant Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free (ab218200)

Overview

  • Product name

    Anti-JNK1+JNK2+JNK3 antibody [EPR18841-95] - BSA and Azide free
    See all JNK1+JNK2+JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR18841-95] to JNK1+JNK2+JNK3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment aa 150-400. The exact sequence is proprietary. SwissProt ID: P45984 = JNK2 and P53779 = JNK3.
    Database link: P45983

  • Positive control

    • WB: Human JNK1, JNK2 and JNK3 full length recombinant proteins; Human fetal liver, fetal heart and fetal kidney lysates; Jurkat, HeLa, K562, MCF7, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain, kidney and spleen lysates. ICC/IF: HeLa and NIH/3T3 cell lines. Flow Cyt: Jurkat and HeLa cell lines. IP: HeLa whole cell lysate.
  • General notes

    Ab218200 is the carrier-free version of ab208035. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab218200 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab218200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 46-54 kDa (predicted molecular weight: 48, 53 kDa).

Target

  • Function

    Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications

    Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • C Jun kinase 2 antibody
    • c Jun N terminal kinase 1 antibody
    • c Jun N terminal kinase 2 antibody
    • c Jun N terminal kinase 3 antibody
    • c-Jun N-terminal kinase 1 antibody
    • JNK 46 antibody
    • JNK 55 antibody
    • JNK antibody
    • JNK-46 antibody
    • JNK1 antibody
    • JNK1+2+3 antibody
    • JNK1/2/3 antibody
    • JNK1A2 antibody
    • JNK2 antibody
    • JNK21B1/2 antibody
    • JNK2A antibody
    • JNK2ALPHA antibody
    • JNK2B antibody
    • JNK2BETA antibody
    • JNK3 alpha protein kinase antibody
    • JNK3 antibody
    • JNK3A antibody
    • Jun kinase antibody
    • JUN N terminal kinase antibody
    • MAP kinase 10 antibody
    • MAP kinase 8 antibody
    • MAP kinase 9 antibody
    • MAP kinase p49 3F12 antibody
    • MAPK 10 antibody
    • MAPK 8 antibody
    • MAPK 9 antibody
    • MAPK10 antibody
    • mapk8 antibody
    • MAPK9 antibody
    • Mitogen activated protein kinase 10 antibody
    • Mitogen activated protein kinase 8 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
    • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
    • Mitogen activated protein kinase 9 antibody
    • Mitogen-activated protein kinase 8 antibody
    • MK08_HUMAN antibody
    • p493F12 antibody
    • p54a antibody
    • p54aSAPK antibody
    • p54bSAPK antibody
    • PRKM10 antibody
    • PRKM8 antibody
    • PRKM9 antibody
    • SAPK antibody
    • SAPK(beta) antibody
    • SAPK1 antibody
    • SAPK1a antibody
    • SAPK1b antibody
    • SAPK1c antibody
    • Stress activated protein kinase 1 antibody
    • Stress activated protein kinase 1a antibody
    • Stress activated protein kinase 1b antibody
    • Stress activated protein kinase 1c antibody
    • Stress activated protein kinase beta antibody
    • Stress activated protein kinase JNK1 antibody
    • Stress activated protein kinase JNK2 antibody
    • Stress activated protein kinase JNK3 antibody
    • Stress-activated protein kinase 1 antibody
    • Stress-activated protein kinase 1c antibody
    • Stress-activated protein kinase JNK1 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm and weak nuclear staining on HeLa cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab208035 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasm staining on NIH/3T3 cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab208035 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.

    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling JNK1+JNK2+JNK3 with ab208035 at 1/100 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat anti-Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

  • JNK1+JNK2+JNK3 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab208035 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab208035 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate, 10ug (Input).

    Lane 2: ab208035 IP in HeLa whole cell lysate.

    Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208035 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208035).

References

ab218200 has not yet been referenced specifically in any publications.

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