Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572)


  • Product name
    Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y]
    See all JNK1+JNK2+JNK3 primary antibodies
  • Description
    Rabbit monoclonal [EP1597Y] to JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223)
  • Host species
  • Specificity
    Detects JNK1 (pY185), JNK2 (pY185) and JNK3 (pY223).
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phospho-peptide corresponding to residues surrounding tyrosine 223 of Human JNK3.

  • Positive control
    • Lysate from UV-treated Hek293 cells
  • General notes

    A trial size is available to purchase for this antibody.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab76572 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1. In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy. Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone. Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH.
      JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.
    • Sequence similarities
      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain
      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • C Jun kinase 2 antibody
      • c Jun N terminal kinase 1 antibody
      • c Jun N terminal kinase 2 antibody
      • c Jun N terminal kinase 3 antibody
      • c-Jun N-terminal kinase 1 antibody
      • JNK 46 antibody
      • JNK 55 antibody
      • JNK antibody
      • JNK-46 antibody
      • JNK1 antibody
      • JNK1A2 antibody
      • JNK2 antibody
      • JNK21B1/2 antibody
      • JNK2A antibody
      • JNK2ALPHA antibody
      • JNK2B antibody
      • JNK2BETA antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • Jun kinase antibody
      • JUN N terminal kinase antibody
      • MAP kinase 10 antibody
      • MAP kinase 8 antibody
      • MAP kinase 9 antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • MAPK 8 antibody
      • MAPK 9 antibody
      • MAPK10 antibody
      • mapk8 antibody
      • MAPK9 antibody
      • Mitogen activated protein kinase 10 antibody
      • Mitogen activated protein kinase 8 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody
      • Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody
      • Mitogen activated protein kinase 9 antibody
      • Mitogen-activated protein kinase 8 antibody
      • MK08_HUMAN antibody
      • p493F12 antibody
      • p54a antibody
      • p54aSAPK antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • PRKM8 antibody
      • PRKM9 antibody
      • SAPK antibody
      • SAPK(beta) antibody
      • SAPK1 antibody
      • SAPK1a antibody
      • SAPK1b antibody
      • SAPK1c antibody
      • Stress activated protein kinase 1 antibody
      • Stress activated protein kinase 1a antibody
      • Stress activated protein kinase 1b antibody
      • Stress activated protein kinase 1c antibody
      • Stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK1 antibody
      • Stress activated protein kinase JNK2 antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase 1c antibody
      • Stress-activated protein kinase JNK1 antibody
      see all


    • All lanes : Anti-JNK1+JNK2+JNK3 (phospho Y185 + Y185 + Y223) antibody [EP1597Y] (ab76572) at 1/20000 dilution

      Lane 1 : lysates from untreated Hek293 cells
      Lane 2 : lysates from Hek293 cells treated with UV

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


    This product has been referenced in:
    • You S  et al. Tunicamycin inhibits colon carcinoma growth and aggressiveness via modulation of the ERK-JNK-mediated AKT/mTOR signaling pathway. Mol Med Rep 17:4203-4212 (2018). Read more (PubMed: 29344654) »
    • Sánchez-Rodríguez C  et al. Immunomodulatory effect of Polypodium leucotomos (Anapsos) in child palatine tonsil model. Int J Pediatr Otorhinolaryngol 107:56-61 (2018). Read more (PubMed: 29501312) »

    See all 14 Publications for this product

    Customer reviews and Q&As

    Western blot
    Rat Cell lysate - whole cell (Primary rat hepatocyte)
    Gel Running Conditions
    Reduced Denaturing (9%)
    Loading amount
    20 µg
    1mM paracetamol
    Primary rat hepatocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Dr. Philip Probert

    Verified customer

    Submitted Jun 03 2016


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