Anti-JNK2 antibody (ab227217)
Key features and details
- Rabbit polyclonal to JNK2
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-JNK2 antibody
See all JNK2 primary antibodies -
Description
Rabbit polyclonal to JNK2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Recombinant fragment within Human JNK2 (internal sequence). The exact sequence is proprietary.
Database link: P45984 -
Positive control
- WB: HeLa and NCI-H1299 whole cell lysates; Mouse brain tissue lysate. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. |
Target
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Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. - Information by UniProt
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Database links
- Entrez Gene: 5601 Human
- Entrez Gene: 26420 Mouse
- Entrez Gene: 50658 Rat
- Omim: 602896 Human
- SwissProt: P45984 Human
- SwissProt: Q9WTU6 Mouse
- SwissProt: P49186 Rat
- Unigene: 484371 Human
see all -
Alternative names
- c Jun kinase 2 antibody
- C Jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 2 antibody
see all
Images
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Western blot - Anti-JNK2 antibody (ab227217)Anti-JNK2 antibody (ab227217) at 1/2000 dilution + Mouse brain tissue lysate at 50 µg10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-JNK2 antibody (ab227217)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for JNK2 (green) using ab227217 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342.
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Western blot - Anti-JNK2 antibody (ab227217)All lanes : Anti-JNK2 antibody (ab227217) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227217 has not yet been referenced specifically in any publications.
