Anti-JNK2 antibody (ab227217)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-JNK2 antibody
See all JNK2 primary antibodies -
Description
Rabbit polyclonal to JNK2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
Recombinant fragment within Human JNK2 (internal sequence). The exact sequence is proprietary.
Database link: P45984 -
Positive control
- WB: HeLa and NCI-H1299 whole cell lysates; Mouse brain tissue lysate. ICC/IF: HeLa cells.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab227217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. |
Target
-
Function
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells.
JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Autophosphorylated in vitro. - Information by UniProt
-
Database links
- Entrez Gene: 5601 Human
- Entrez Gene: 26420 Mouse
- Entrez Gene: 50658 Rat
- Omim: 602896 Human
- SwissProt: P45984 Human
- SwissProt: Q9WTU6 Mouse
- SwissProt: P49186 Rat
- Unigene: 484371 Human
see all -
Alternative names
- c Jun kinase 2 antibody
- C Jun N terminal kinase 2 antibody
- c-Jun N-terminal kinase 2 antibody
see all
Images
-
Western blot - Anti-JNK2 antibody (ab227217)Anti-JNK2 antibody (ab227217) at 1/2000 dilution + Mouse brain tissue lysate at 50 µg10% SDS-PAGE gel.
-
Immunocytochemistry/ Immunofluorescence - Anti-JNK2 antibody (ab227217)Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for JNK2 (green) using ab227217 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342.
-
Western blot - Anti-JNK2 antibody (ab227217)All lanes : Anti-JNK2 antibody (ab227217) at 1/1000 dilution
Lane 1 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.10% SDS-PAGE gel.
References
ab227217 has not yet been referenced specifically in any publications.
