Overview

  • Product name

    Anti-JNK3 antibody [EPR5493(2)]
    See all JNK3 primary antibodies
  • Description

    Rabbit monoclonal [EPR5493(2)] to JNK3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human JNK3 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and Human heart lysates; 293 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.
    • Tissue specificity

      Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
    • Involvement in disease

      Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.
    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications

      Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • c Jun kinase 3 antibody
      • c-Jun N-terminal kinase 3 antibody
      • cJun N terminal kinase 3 antibody
      • FLJ12099 antibody
      • FLJ33785 antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • MAP kinase 10 antibody
      • MAP kinase antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • Mapk10 antibody
      • MGC50974 antibody
      • mitogen activated protein kinase 10 antibody
      • Mitogen-activated protein kinase 10 antibody
      • MK10_HUMAN antibody
      • p493F12 antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • protein kinase mitogen activated 10 antibody
      • SAPK1b antibody
      • Stress activated protein kinase 1b antibody
      • stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase JNK3 antibody
      see all

    Images

    • All lanes : Anti-JNK3 antibody [EPR5493(2)] (ab126591) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa

    • Overlay histogram showing SHSY-5Y cells stained with ab126591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126591, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • ab126591 at 1/100 dilution staining JNK3 in 293 cells by immunofluorescence.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126591 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    The immunogen of this antibody corresponds to a region within amino acids 400-450 of the protein. This has 100% homology with Isoform Alpha-2 (53 kDa) and Isoform 3 (48 kDa) but no homology with Isoform Alpha-1 and Isoform 4. Please see the uniprot entry for this protein for more information on the isoforms: http://www.uniprot.org/uniprot/P53779

    This antibody can therefore detect bands at 53 kDa and 48 kDa which would explain the results you have obtained.

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