Recombinant
RabMAb

Recombinant Anti-JNK3 antibody [EPR5493(2)] (ab126591)

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Overview

  • Product name

    Anti-JNK3 antibody [EPR5493(2)]
    See all JNK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5493(2)] to JNK3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human JNK3 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and Human heart lysates; 293 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab126591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.

    • Tissue specificity

      Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

    • Involvement in disease

      Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.

    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    • Post-translational
      modifications

      Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • c Jun kinase 3 antibody
      • c-Jun N-terminal kinase 3 antibody
      • cJun N terminal kinase 3 antibody
      • FLJ12099 antibody
      • FLJ33785 antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • MAP kinase 10 antibody
      • MAP kinase antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • Mapk10 antibody
      • MGC50974 antibody
      • mitogen activated protein kinase 10 antibody
      • Mitogen-activated protein kinase 10 antibody
      • MK10_HUMAN antibody
      • p493F12 antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • protein kinase mitogen activated 10 antibody
      • SAPK1b antibody
      • Stress activated protein kinase 1b antibody
      • stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase JNK3 antibody
      see all

    Images

    • Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      All lanes : Anti-JNK3 antibody [EPR5493(2)] (ab126591) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa

    • Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Overlay histogram showing SHSY-5Y cells stained with ab126591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126591, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)

      ab126591 at 1/100 dilution staining JNK3 in 293 cells by immunofluorescence.

    • Other - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Other - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Yang XL & Gao CM c-Jun N-terminal kinase 3 signalling serves a potential role as a biomarker for determining the pathogenesis of Parkinson's disease. Mol Med Rep 17:3255-3259 (2018). Read more (PubMed: 29257285) »
    See 1 Publication for this product

    Publishing research using ab126591? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hi, I used the antibody (126591) from Abcam that recognizes MAPK10 (JNK3). Based on your sheet, there should be only one band (53KD) in Hela cells. However, I do find a smaller size band (˜46KD). Not sure if this is one isoform of JNK3.

    Read More
    Answer

    The immunogen of this antibody corresponds to a region within amino acids 400-450 of the protein. This has 100% homology with Isoform Alpha-2 (53 kDa) and Isoform 3 (48 kDa) but no homology with Isoform Alpha-1 and Isoform 4. Please see the uniprot entry for this protein for more information on the isoforms: http://www.uniprot.org/uniprot/P53779

    This antibody can therefore detect bands at 53 kDa and 48 kDa which would explain the results you have obtained.

    Read More

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