Recombinant
RabMAb

Recombinant Anti-JNK3 antibody [EPR5493(2)] (ab126591)

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Overview

  • Product name

    Anti-JNK3 antibody [EPR5493(2)]
    See all JNK3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5493(2)] to JNK3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human JNK3 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, SH-SY5Y and Human heart lysates; 293 cells.

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity.

    • Tissue specificity

      Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

    • Involvement in disease

      Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation.

    • Sequence similarities

      Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.

    • Domain

      The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    • Post-translational
      modifications

      Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • c Jun kinase 3 antibody
      • c-Jun N-terminal kinase 3 antibody
      • cJun N terminal kinase 3 antibody
      • FLJ12099 antibody
      • FLJ33785 antibody
      • JNK3 alpha protein kinase antibody
      • JNK3 antibody
      • JNK3A antibody
      • MAP kinase 10 antibody
      • MAP kinase antibody
      • MAP kinase p49 3F12 antibody
      • MAPK 10 antibody
      • Mapk10 antibody
      • MGC50974 antibody
      • mitogen activated protein kinase 10 antibody
      • Mitogen-activated protein kinase 10 antibody
      • MK10_HUMAN antibody
      • p493F12 antibody
      • p54bSAPK antibody
      • PRKM10 antibody
      • protein kinase mitogen activated 10 antibody
      • SAPK1b antibody
      • Stress activated protein kinase 1b antibody
      • stress activated protein kinase beta antibody
      • Stress activated protein kinase JNK3 antibody
      • Stress-activated protein kinase JNK3 antibody
      see all

    Images

    • Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Western blot - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      All lanes : Anti-JNK3 antibody [EPR5493(2)] (ab126591) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : SH-SY5Y cell lysate
      Lane 4 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 53 kDa

    • Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Flow Cytometry - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Overlay histogram showing SHSY-5Y cells stained with ab126591 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab126591, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Immunocytochemistry/ Immunofluorescence - Anti-JNK3 antibody [EPR5493(2)] (ab126591)

      ab126591 at 1/100 dilution staining JNK3 in 293 cells by immunofluorescence.

    • Other - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Other - Anti-JNK3 antibody [EPR5493(2)] (ab126591)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab126591 has not yet been referenced specifically in any publications.

    Publishing research using ab126591? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Question

    Hi, I used the antibody (126591) from Abcam that recognizes MAPK10 (JNK3). Based on your sheet, there should be only one band (53KD) in Hela cells. However, I do find a smaller size band (˜46KD). Not sure if this is one isoform of JNK3.

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    Answer

    The immunogen of this antibody corresponds to a region within amino acids 400-450 of the protein. This has 100% homology with Isoform Alpha-2 (53 kDa) and Isoform 3 (48 kDa) but no homology with Isoform Alpha-1 and Isoform 4. Please see the uniprot entry for this protein for more information on the isoforms: http://www.uniprot.org/uniprot/P53779

    This antibody can therefore detect bands at 53 kDa and 48 kDa which would explain the results you have obtained.

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