Key features and details
- Rabbit polyclonal to JOSD1
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-JOSD1 antibody
DescriptionRabbit polyclonal to JOSD1
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Recombinant fragment corresponding to Human JOSD1 aa 44-188. (BC015026)
- WB: A549 and HepG2 cell lysates. IHC: human prostate carcinoma tissue
This product was previously labelled as JOSD1, Josephin-1
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab118221 is purified by a protein affinity column.
Our Abpromise guarantee covers the use of ab118221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/100 - 1/500.|
|ELISA||1/20000 - 1/80000.|
FunctionHas low protease activity towards poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.
Sequence similaritiesContains 1 Josephin domain.
- Information by UniProt
- dJ508I15.2 antibody
- JOS1_HUMAN antibody
- Josd1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labeling JOSD1 with ab118221 at a dilution of 1/100.
All lanes : Anti-JOSD1 antibody (ab118221) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Predicted band size: 23 kDa
ab118221 has been referenced in 1 publication.
- Wu X et al. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1. Cell Death Differ N/A:N/A (2019). PubMed: 31043700