Anti-JOSD1 antibody (ab118221)
Key features and details
- Rabbit polyclonal to JOSD1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-JOSD1 antibody -
Description
Rabbit polyclonal to JOSD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human JOSD1 aa 44-188. (BC015026)
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Positive control
- WB: A549 and HepG2 cell lysates. IHC: human prostate carcinoma tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab118221 is purified by a protein affinity column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab118221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IHC-P |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IHC-P
1/100 - 1/500. |
Target
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Function
Has low protease activity towards poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme. -
Sequence similarities
Contains 1 Josephin domain. - Information by UniProt
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Database links
- Entrez Gene: 9929 Human
- Entrez Gene: 74158 Mouse
- Entrez Gene: 315134 Rat
- SwissProt: Q15040 Human
- SwissProt: Q9DBJ6 Mouse
- SwissProt: Q5BJY4 Rat
- Unigene: 3094 Human
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Alternative names
- dJ508I15.2 antibody
- JOS1_HUMAN antibody
- Josd1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labeling JOSD1 with ab118221 at a dilution of 1/100.
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All lanes : Anti-JOSD1 antibody (ab118221) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : A549 cell lysate
Predicted band size: 23 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab118221 has been referenced in 1 publication.
- Wu X et al. JOSD1 inhibits mitochondrial apoptotic signalling to drive acquired chemoresistance in gynaecological cancer by stabilizing MCL1. Cell Death Differ N/A:N/A (2019). PubMed: 31043700