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Anti-JP-1 antibody (ab195312)

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Immunoprecipitation - Anti-JP-1 antibody (ab195312)
  • Western blot - Anti-JP-1 antibody (ab195312)

Key features and details

  • Rabbit polyclonal to JP-1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-JP-1 antibody
    See all JP-1 primary antibodies
  • Description

    Rabbit polyclonal to JP-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Rhesus monkey, Gorilla, Opossum, Elephant
  • Immunogen

    Synthetic peptide within Human JP-1 aa 450-500 (internal sequence). The exact sequence is proprietary.
    Sequence:

    KESPHFYRKGTTPPRSPEASPKHSHSPASSPKPLKKQNPSSGARLNQDKR S


    Database link: Q9HDC5
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa and 293T cell lysates.
  • General notes

    Previously labelled as JPH1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)

Applications

Our Abpromise guarantee covers the use of ab195312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 72 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH1 contributes to the construction of the skeletal muscle triad by linking the t-tubule (transverse-tubule) and SR (sarcoplasmic reticulum) membranes.
  • Tissue specificity

    Abundantly expressed in skeletal muscle. Very low levels in heart.
  • Sequence similarities

    Belongs to the junctophilin family.
    Contains 7 MORN repeats.
  • Domain

    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In skeletal muscle cells, it is predominantly localized at the junction of the A and I bands.
  • Target information above from: UniProt accession Q9HDC5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 56704 Human
    • Omim: 605266 Human
    • SwissProt: Q9HDC5 Human
    • Unigene: 657367 Human
    • Alternative names

      • DKFZp762L0313 antibody
      • JP 1 antibody
      • JP-1 antibody
      • JP1 antibody
      • JPH 1 antibody
      • JPH1 antibody
      • JPH1_HUMAN antibody
      • Junctophilin 1 antibody
      • Junctophilin type 1 antibody
      • Junctophilin type1 antibody
      • Junctophilin-1 antibody
      • Junctophilin1 antibody
      • Mitsugumin 72 antibody
      • Mitsugumin72 antibody
      see all

    Images

    • Immunoprecipitation - Anti-JP-1 antibody (ab195312)
      Immunoprecipitation - Anti-JP-1 antibody (ab195312)

      Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer.  Antibodies: ab195312 used for IP at 6 µg per reaction. For blotting immunoprecipitated Junctophilin 1, ab195312 was used at 1 µg/mL.

    • Western blot - Anti-JP-1 antibody (ab195312)
      Western blot - Anti-JP-1 antibody (ab195312)
      All lanes : Anti-JP-1 antibody (ab195312) at 0.1 µg/ml

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 50 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 72 kDa


      Exposure time: 3 minutes

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab195312? Please let us know so that we can cite the reference in this datasheet.

    ab195312 has not yet been referenced specifically in any publications.

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