Overview

  • Product name

  • Description

    Rabbit polyclonal to JP-2
  • Host species

    Rabbit
  • Specificity

    Multiple isoforms of JPH2 are known to exist.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human JP-2 (C terminal).

  • Positive control

    • Human Heart and Skeletal Muscle. Mouse brain tissue lysate.
  • General notes

    Protein previously labeled as Junctophilin-2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 75 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic/sarcoplasmic reticulum. Contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.
  • Tissue specificity

    Specifically expressed in skeletal muscle and heart.
  • Involvement in disease

    Defects in JPH2 are the cause of cardiomyopathy familial hypertrophic type 17 (CMH17) [MIM:613873]. CMH17 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities

    Belongs to the junctophilin family.
    Contains 8 MORN repeats.
  • Domain

    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ40969 antibody
    • JP-2 antibody
    • JP2 antibody
    • Jph2 antibody
    • JPH2_HUMAN antibody
    • Junctophilin 2 antibody
    • Junctophilin type 2 antibody
    • Junctophilin-2 antibody
    • OTTHUMP00000031651 antibody
    • OTTHUMP00000031652 antibody
    see all

Images

  • Lane 1 : Anti-JP-2 antibody (ab110056) at 1 µg/ml
    Lane 2 : Anti-JP-2 antibody (ab110056) at 2 µg/ml

    All lanes : Mouse brain tissue lysate

    Predicted band size: 75 kDa

  • ab110056 at 5 µg/ml staining JP-2 in Formalin-Fixed, Paraffin-Embedded Human Heart by Immunohistochemistry.

  • ab110056 at 5 µg/ml staining JP-2 in Formalin-Fixed, Paraffin-Embedded Human Skeletal Muscle by Immunohistochemistry.

References

ab110056 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Heart)
Permeabilization
Yes - 0.5% Triton @ RT 3H
Specification
Heart
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde

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Verified customer

Submitted Apr 25 2018

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