Overview

  • Product name

  • Description

    Rabbit polyclonal to JP-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human JP-2 (C terminal).
    (Peptide available as ab110064)

  • Positive control

    • Mouse brain tissue lysate
  • General notes

    Protein previously labeled as Junctophilin-2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Affinity chromatography purified via peptide column
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 74 kDa.Can be blocked with JP-2 peptide (ab110064).
ELISA Use at an assay dependent dilution.

Target

  • Function

    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic/sarcoplasmic reticulum. Contributes to the construction of skeletal muscle triad junctions, and plays an essential role in heart development.
  • Tissue specificity

    Specifically expressed in skeletal muscle and heart.
  • Involvement in disease

    Defects in JPH2 are the cause of cardiomyopathy familial hypertrophic type 17 (CMH17) [MIM:613873]. CMH17 is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • Sequence similarities

    Belongs to the junctophilin family.
    Contains 8 MORN repeats.
  • Domain

    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In heart cells, it predominantly associates along Z lines within myocytes. In skeletal muscle, it is specifically localized at the junction of A and I bands.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ40969 antibody
    • JP-2 antibody
    • JP2 antibody
    • Jph2 antibody
    • JPH2_HUMAN antibody
    • Junctophilin 2 antibody
    • Junctophilin type 2 antibody
    • Junctophilin-2 antibody
    • OTTHUMP00000031651 antibody
    • OTTHUMP00000031652 antibody
    see all

Images

  • Lane 1 : Anti-JP-2 antibody (ab79071) at 1 µg/ml
    Lane 2 : Anti-JP-2 antibody (ab79071) at 2 µg/ml

    All lanes : mouse brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa
    Additional bands at: 64 kDa (possible isoform)

References

This product has been referenced in:

  • Bennett HJ  et al. Human junctophilin-2 undergoes a structural rearrangement upon binding PtdIns(3,4,5)P3 and the S101R mutation identified in hypertrophic cardiomyopathy obviates this response. Biochem J 456:205-17 (2013). Read more (PubMed: 24001019) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Sheep Tissue lysate - whole (Cardiac)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
10 µg
Specification
Cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 15 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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