Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH1 contributes to the construction of the skeletal muscle triad by linking the t-tubule (transverse-tubule) and SR (sarcoplasmic reticulum) membranes.
Abundantly expressed in skeletal muscle. Very low levels in heart.
Belongs to the junctophilin family. Contains 7 MORN repeats.
The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
Cell membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic/sarcoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane. In skeletal muscle cells, it is predominantly localized at the junction of the A and I bands.
Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using RIPA lysis buffer. Antibodies: ab195312 used for IP at 6 µg per reaction. For blotting immunoprecipitated Junctophilin 1, ab195312 was used at 1 µg/mL.
Western blot - Anti-JPH1 antibody (ab195312)
All lanes : Anti-JPH1 antibody (ab195312) at 0.1 µg/ml
Lane 1 : HeLa cell lysate Lane 2 : 293T cell lysate Lane 3 : Jurkat cell lysate