Key features and details
- Rabbit polyclonal to JPH3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-JPH3 antibody
DescriptionRabbit polyclonal to JPH3
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A synthetic 18 amino acid peptide near the carboxy terminus of human JPH3 (protein accession number: NP_065706)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity chromatography purified via peptide column
Our Abpromise guarantee covers the use of ab79063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.|
FunctionContributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic reticulum. May play an active role in certain neurons involved in motor coordination.
Tissue specificitySpecifically expressed in brain.
Involvement in diseaseDefects in JPH3 are the cause of Huntington disease-like type 2 (HDL2) [MIM:606438]. Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial adult onset chorea and subcortical dementia that usually begin during the fourth decade of life.
Sequence similaritiesBelongs to the junctophilin family.
Contains 8 MORN repeats.
DomainThe MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
Cellular localizationCell membrane. Endoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane.
- Information by UniProt
- JP-3 antibody
- JP3 antibody
- JPH3 antibody
All lanes : Anti-JPH3 antibody (ab79063) at 1 µg/ml
Lane 1 : Daudi cell lysate
Lane 2 : Daudi cell lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Immunofluorescence of JPH3 in Human Brain cells using ab79063 at 20 ug/ml.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab79063 has not yet been referenced specifically in any publications.