Overview

  • Product name

    Anti-JPH3 antibody
  • Description

    Rabbit polyclonal to JPH3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic 18 amino acid peptide near the carboxy terminus of human JPH3 (protein accession number: NP_065706)

  • Positive control

    • Daudi cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab79063 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 81 kDa.
ELISA Use at an assay dependent dilution.

Target

  • Function

    Contributes to the stabilization of the junctional membrane complexes, which are common to excitable cells and mediate cross-talk between cell surface and intracellular ion channels. Probably acts by anchoring the plasma membrane and endoplasmic reticulum. May play an active role in certain neurons involved in motor coordination.
  • Tissue specificity

    Specifically expressed in brain.
  • Involvement in disease

    Defects in JPH3 are the cause of Huntington disease-like type 2 (HDL2) [MIM:606438]. Huntington disease (HD) is a neurodegenerative disorder resulting primarily from the loss of medium spiny projection neurons in the striatum, especially in the caudate nucleus, and, to a lesser extent, atrophy of mesencephalic and cortical structures. The typical clinical picture of HD combines familial adult onset chorea and subcortical dementia that usually begin during the fourth decade of life.
  • Sequence similarities

    Belongs to the junctophilin family.
    Contains 8 MORN repeats.
  • Domain

    The MORN (membrane occupation and recognition nexus) repeats contribute to the plasma membrane binding, possibly by interacting with phospholipids.
  • Cellular localization

    Cell membrane. Endoplasmic reticulum membrane. Localized predominantly on the plasma membrane. The transmembrane domain is anchored in endoplasmic reticulum membrane, while the N-terminal part associates with the plasma membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • JP-3 antibody
    • JP3 antibody
    • JPH3 antibody
    • JPH3_HUMAN antibody
    • Junctophilin 3 antibody
    • Junctophilin type 3 antibody
    • Junctophilin-3 antibody
    • TNRC22 antibody
    • Trinucleotide repeat-containing gene 22 protein antibody
    see all

Images

  • All lanes : Anti-JPH3 antibody (ab79063) at 1 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 81 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence of JPH3 in Human Brain cells using ab79063 at 20 ug/ml.

References

ab79063 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx with ab81178. The estimated delivery date is xxx.

To check the status of the order please contact our Customer Service team and reference this number.

Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

Here is the diiscount code for trying the antibody ab81778 in mouse.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab81178 in mouse. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for mouseand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

I wish you the best of luck with your research.

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