Product nameAnti-JunB antibody
See all JunB primary antibodies
DescriptionRabbit polyclonal to JunB
Tested applicationsSuitable for: IP, IHC-Pmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human JunB aa 40-80. The exact sequence is proprietary.
Database link: P17275
- IHC-P: Human breast carcinoma tissue. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionTranscription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
modificationsUbiquitinated by ITCH, leading to its degradation.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling JunB with ab264302 at 1/5,000 dilution. Detection: DAB.
JunB was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264302 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using a different antibody.
Lane 1: ab264302 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264302 has not yet been referenced specifically in any publications.