Key features and details
- Rabbit polyclonal to JunB
- Suitable for: WB, ChIP, IP
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-JunB antibody
See all JunB primary antibodies
DescriptionRabbit polyclonal to JunB
Tested applicationsSuitable for: WB, ChIP, IPmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Human
- WB: uninduced and induced L929 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab3525 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|ChIP||Use at an assay dependent concentration.
Dilution = 1-3 ul
|IP||Use at an assay dependent concentration.
Dilution: assay dependent
FunctionTranscription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
modificationsUbiquitinated by ITCH, leading to its degradation.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
All lanes : Anti-JunB antibody (ab3525)
Lane 1 : L929 cell extract
Lane 2 : Extract from L929 cells treated with dexamethasone
Predicted band size: 38 kDa
Observed band size: 38 kDa
Additional bands at: 50 kDa (possible cross reactivity)
ab3525 in Western blot on L929 cells. There is the specific band at 38 kDa. There are also non-specific bands at 50 kDa and a very faint one at 40 kDa.
ab3525 has been referenced in 1 publication.
- Liu B et al. Paeoniflorin Inhibits Mesangial Cell Proliferation and Inflammatory Response in Rats With Mesangial Proliferative Glomerulonephritis Through PI3K/AKT/GSK-3ß Pathway. Front Pharmacol 10:978 (2019). PubMed: 31551783