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  1. Link

    junb-antibody-ab87649.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper Leucine Zipper
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Anti-JunB antibody (ab87649)

  • Datasheet
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  • Western blot - Anti-JunB antibody (ab87649)

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Primary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-JunB antibody
    See all JunB primary antibodies
  • Description

    Goat polyclonal to JunB
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Common marmoset
  • Immunogen

    Synthetic peptide with sequence

    C-TLKAENAGLSSTAG

    , corresponding to internal sequence amino acids 302 - 315 of Human JunB, with a Cysteine residue linker (NP_002220.1.)

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Huamn breast cancer lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab87649 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human JunB protein (ab152484)

Applications

Our Abpromise guarantee covers the use of ab87649 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 36 kDa.

Target

  • Function

    Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
  • Sequence similarities

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications

    Ubiquitinated by ITCH, leading to its degradation.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P17275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 514246 Cow
    • Entrez Gene: 484927 Dog
    • Entrez Gene: 3726 Human
    • Entrez Gene: 16477 Mouse
    • Entrez Gene: 24517 Rat
    • Omim: 165161 Human
    • SwissProt: Q0VBZ5 Cow
    • SwissProt: P17275 Human
    • SwissProt: P09450 Mouse
    • SwissProt: P24898 Rat
    • Unigene: 25292 Human
    • Unigene: 1167 Mouse
    • Unigene: 470093 Mouse
    • Unigene: 15806 Rat
    see all
  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • Jun B antibody
    • Jun B proto oncogene antibody
    • Jun B protooncogene antibody
    • Junb antibody
    • JunB proto oncogene antibody
    • JunB protoncogene 9 antibody
    • JunB protooncogene antibody
    • JUNB_HUMAN antibody
    • Transcription factor jun B antibody
    • Transcription factor jun-B antibody
    • Transcription factor junB antibody
    see all

Images

  • Western blot - Anti-JunB antibody (ab87649)
    Western blot - Anti-JunB antibody (ab87649)
    Anti-JunB antibody (ab87649) at 0.3 µg/ml + Human breast cancer cell lysate (in RIPA buffer) at 35 µg/ml

    Developed using the ECL technique.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa



    Primary incubation was 1 hour.

Protocols

  • Western blot protocols

Datasheets and documents

    • Datasheet
  • References

    ab87649 has not yet been referenced specifically in any publications.

    Publishing research using ab87649? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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