Anti-JunB antibody (ab87649)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-JunB antibody
See all JunB primary antibodies -
Description
Goat polyclonal to JunB -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Common marmoset
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Immunogen
Synthetic peptide with sequence
C-TLKAENAGLSSTAG
, corresponding to internal sequence amino acids 302 - 315 of Human JunB, with a Cysteine residue linker (NP_002220.1.) -
Positive control
- Huamn breast cancer lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab87649 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab87649 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 36 kDa. |
Target
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Function
Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsUbiquitinated by ITCH, leading to its degradation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 514246 Cow
- Entrez Gene: 484927 Dog
- Entrez Gene: 3726 Human
- Entrez Gene: 16477 Mouse
- Entrez Gene: 24517 Rat
- Omim: 165161 Human
- SwissProt: Q0VBZ5 Cow
- SwissProt: P17275 Human
see all -
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
see all
Images
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Western blot - Anti-JunB antibody (ab87649)Anti-JunB antibody (ab87649) at 0.3 µg/ml + Human breast cancer cell lysate (in RIPA buffer) at 35 µg/ml
Developed using the ECL technique.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
References
ab87649 has not yet been referenced specifically in any publications.
