Overview

  • Product name

    Anti-JunB antibody [EPR6518]
    See all JunB primary antibodies
  • Description

    Rabbit monoclonal [EPR6518] to JunB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human JunB (N terminal). The exact sequence is proprietary.
    Database link: P17275

  • Positive control

    • WB: MCF7, Human tonsil, Human uterus cancer, mouse uterus, rat lung, rat spleen, HeLa and HACAT lysates ICC/IF: HeLa, and MCF7 cells. IP: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 36 kDa.

For unpurified use at 1/1000 - 1/10000.

IP 1/10 - 1/100.
ICC/IF 1/200.

For unpurified use at 1/250- 1/500.

  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
    • Sequence similarities

      Belongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Post-translational
      modifications

      Ubiquitinated by ITCH, leading to its degradation.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun B antibody
      • Jun B proto oncogene antibody
      • Jun B protooncogene antibody
      • Junb antibody
      • JunB proto oncogene antibody
      • JunB protoncogene 9 antibody
      • JunB protooncogene antibody
      • JUNB_HUMAN antibody
      • Transcription factor jun B antibody
      • Transcription factor jun-B antibody
      • Transcription factor junB antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling JunB with purified ab128878 at 1:200 dilution (9.1 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 �g/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 �g/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/200 dilution (Purified)

      Lane 1 : Rat lung lysates
      Lane 2 : Rat spleen lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : Mouse uterus lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?

    • Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Purified) + Human uterus lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 36 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?

    • ab128878 (purified) at 1:100 dilution (2 µg) immunoprecipitating JunB in HeLa whole cell lysates.
      Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates 10ug
      Lane 2 (+): ab128878 & HeLa whole cell lysates
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab128878 in HeLa whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP)�(ab131366) was used at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.
    • All lanes : Anti-JunB antibody [EPR6518] (ab128878) at 1/1000 dilution (Unpurified)

      Lane 1 : MCF-7 lysate
      Lane 2 : Human tonsil lysate
      Lane 3 : Human uterus cancer lysate
      Lane 4 : HeLa lysate
      Lane 5 : HACAT lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 36 kDa

    • Unpurified ab128878, at a 1/250 dilution, staining JunB in MCF7 (Human breast adenocarcinoma cell line) cells by Immunofluorescence.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Shirali AS  et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). Read more (PubMed: 29615716) »
    • Suwei D  et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . Read more (PubMed: 26503334) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab128878.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up