Overview

  • Product name

    Anti-JunB antibody [EPR6518] (Alexa Fluor® 647)
    See all JunB primary antibodies
  • Description

    Rabbit monoclonal [EPR6518] to JunB (Alexa Fluor® 647)
  • Host species

    Rabbit
  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues near the N-terminal in Human JunB (P17275).

  • Positive control

    • ICC/IF: MCF7 cells
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Applications

Our Abpromise guarantee covers the use of ab200516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200.

Target

  • Function

    Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'.
  • Sequence similarities

    Belongs to the bZIP family. Jun subfamily.
    Contains 1 bZIP domain.
  • Post-translational
    modifications

    Ubiquitinated by ITCH, leading to its degradation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator protein 1 antibody
    • AP 1 antibody
    • AP1 antibody
    • Jun B antibody
    • Jun B proto oncogene antibody
    • Jun B protooncogene antibody
    • Junb antibody
    • JunB proto oncogene antibody
    • JunB protoncogene 9 antibody
    • JunB protooncogene antibody
    • JUNB_HUMAN antibody
    • Transcription factor jun B antibody
    • Transcription factor jun-B antibody
    • Transcription factor junB antibody
    see all

Images

  • ab200516 staining JunB in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab200516 at 1/200 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 2µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

References

ab200516 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mrine Breast Tumor)
Permeabilization
Yes - 15min in PBS-Triton-X 60¯C
Specification
Mrine Breast Tumor
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 28 2017

Application
Flow Cytometry
Sample
Mouse Cell (Mouse Embryonic Fibroblasts)
Permeabilization
Yes - 1xPBS/0.3% Triton-X
Gating Strategy
Alexa647+
Specification
Mouse Embryonic Fibroblasts
Preparation
Cell harvesting/tissue preparation method: Trypsinisation
Sample buffer: 1% BSA in PBS
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 25 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblasts)
Permeabilization
Yes - PBS/0.3% Triton-X for 15min at RT
Specification
Mouse Embryonic Fibroblasts
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 25 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LX2 hepatic stellate cells)
Permeabilization
Yes - PBS/0.3% Triton-X for 15min at RT
Specification
LX2 hepatic stellate cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 25 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Murine Breast Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric Buffer, pH 6
Permeabilization
Yes - 15min in PBS-Triton-X 60¯C
Specification
Murine Breast Tumor
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 25 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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