Product nameJunB overexpression 293T lysate (whole cell)
ab94280 is a 293T cell transfected lysate in which Human JunB has been transiently over-expressed using a pCMV-JunB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
PurityWhole Cell Lysate
BackgroundFunction: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Similarity: Belongs to the bZIP family. Jun subfamily. Contains 1 bZIP domain. PTM: Ubiquitinated by ITCH, leading to its degradation.
Our Abpromise guarantee covers the use of ab94280 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94280 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-JunB antibody (ab90565) at 1/500 dilution
Lane 1 :
JunB overexpression 293T lysate (whole cell) (ab94280)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94280 has not yet been referenced specifically in any publications.