Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886)
Key features and details
- Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Junctional Adhesion Molecule 1/JAM-A antibody
See all Junctional Adhesion Molecule 1/JAM-A primary antibodies -
Description
Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chinese hamster -
Immunogen
Synthetic peptide corresponding to Mouse Junctional Adhesion Molecule 1/JAM-A aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab156888) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa). |
Target
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Function
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 50848 Human
- Entrez Gene: 16456 Mouse
- Entrez Gene: 116479 Rat
- Omim: 605721 Human
- SwissProt: Q9Y624 Human
- SwissProt: O88792 Mouse
- SwissProt: Q9JHY1 Rat
- Unigene: 517293 Human
see all -
Alternative names
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
see all
Images
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All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutesJunctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab125886 has been referenced in 4 publications.
- Wang W et al. NEO100 enables brain delivery of blood?brain barrier impermeable therapeutics. Neuro Oncol 23:63-75 (2021). PubMed: 32877532
- Zhang XY et al. Sishen Pill Maintained Colonic Mucosal Barrier Integrity to Treat Ulcerative Colitis via Rho/ROCK Signaling Pathway. Evid Based Complement Alternat Med 2021:5536679 (2021). PubMed: 34925530
- Ducray HAG et al. Prevention of excessive exercise-induced adverse effects in rats with Bacillus subtilis BSB3. J Appl Microbiol 128:1163-1178 (2020). PubMed: 31814258
- Ducray HAG et al. Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats. J Appl Microbiol N/A:N/A (2019). WB ; Rat . PubMed: 31230390