Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886)

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 1/JAM-A antibody
    See all Junctional Adhesion Molecule 1/JAM-A primary antibodies
  • Description

    Rabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Mouse Junctional Adhesion Molecule 1/JAM-A aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156888)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.

Properties

Applications

Our Abpromise guarantee covers the use of ab125886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).

Target

  • Function

    Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
  • Cellular localization

    Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • CD 321 antibody
    • CD321 antibody
    • CD321 antigen antibody
    • ESTM33 antibody
    • F11 receptor antibody
    • F11R antibody
    • JAM 1 antibody
    • JAM A antibody
    • JAM antibody
    • JAM-1 antibody
    • JAM-A antibody
    • JAM1 antibody
    • JAM1_HUMAN antibody
    • JAMA antibody
    • JCAM antibody
    • Jcam1 antibody
    • Junction adhesion molecule 1 antibody
    • Junction adhesion molecule, mouse, homolog of antibody
    • Junctional adhesion molecule 1 antibody
    • Junctional adhesion molecule A antibody
    • KAT antibody
    • Ly106 antibody
    • PAM 1 antibody
    • PAM-1 antibody
    • PAM1 antibody
    • Platelet adhesion molecule 1 antibody
    • Platelet adhesion molecule antibody
    • Platelet F11 receptor antibody
    • PRO301 antibody
    • UNQ264 antibody
    see all

Images

  • All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/ml

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Brain (Rat) Tissue Lysate
    Lane 3 : Mouse Cortex Tissue Lysate
    Lane 4 : Rat Cortex Tissue Lysate
    Lane 5 : Cerebellum Mouse Tissue Lysate
    Lane 6 : Spinal Cord (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 32 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 53 kDa, 99 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Junctional Adhesion Molecule 1/JAM-A  contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A  is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:

  • Ducray HAG  et al. Yeast fermentate prebiotic improves intestinal barrier integrity during heat stress by modulation of the gut microbiota in rats. J Appl Microbiol N/A:N/A (2019). WB ; Rat . Read more (PubMed: 31230390) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Large Intestine)
Specification
Large Intestine
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 10 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris MOPS)
Loading amount
10 µg
Specification
intestinal tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 20 2015

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