Product nameAnti-Junctional Adhesion Molecule 1/JAM-A antibody
See all Junctional Adhesion Molecule 1/JAM-A primary antibodies
DescriptionRabbit polyclonal to Junctional Adhesion Molecule 1/JAM-A
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Chinese hamster
Synthetic peptide corresponding to Mouse Junctional Adhesion Molecule 1/JAM-A aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab125886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).|
FunctionSeems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- CD 321 antibody
- CD321 antibody
- CD321 antigen antibody
All lanes : Anti-Junctional Adhesion Molecule 1/JAM-A antibody (ab125886) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Mouse Cortex Tissue Lysate
Lane 4 : Rat Cortex Tissue Lysate
Lane 5 : Cerebellum Mouse Tissue Lysate
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 53 kDa, 99 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Junctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.