Mouse, Rat Predicted to work with:
Synthetic peptide corresponding to Mouse Junctional Adhesion Molecule 1/JAM-A aa 50-150 conjugated to keyhole limpet haemocyanin. (Peptide available as ab156888)
This antibody gave a positive signal in the following tissue lysates: Mouse Brain; Rat Brain; Mouse Cortex; Rat Cortex; Mouse Cerebellum; Mouse Spinal Cord.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 32 kDa).
Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.
Belongs to the immunoglobulin superfamily. Contains 2 Ig-like V-type (immunoglobulin-like) domains.
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
Junctional Adhesion Molecule 1/JAM-A contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The predicted molecular weight of Junctional Adhesion Molecule 1/JAM-A is 32 kDa (SwissProt), however we expect to observe a banding pattern around 42 kDa. This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab125886 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.