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Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2
Flow Cyt more details Unsuitable for:
ICC,IHC-P or IP
Mouse, Rat, Human
A synthetic peptide corresponding to residues in Human Junctional Adhesion Molecule 2 (UniProt ID: P57087).
HUVEC, HeLa, JAR and A431 cell lysates, A431 cells
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Is unsuitable for ICC,IHC-P or IP.
May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
Information by UniProt
CD 322 antibody
Western blot - Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)] (ab156586)
All lanes : Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)] (ab156586) at 1/1000 dilution Lane 1 : HUVEC cell lysates Lane 2 : HeLa cell lysates Lane 3 : JAR cell lysates Lane 4 : A431 cell lysates Lysates/proteins at 10 µg per lane. Secondary All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution Predicted band size: 33 kDa
Flow Cytometry - Anti-Junctional Adhesion Molecule 2 antibody [EPR2489(2)] (ab156586)
Flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2 using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"