Recombinant Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)]
See all Junctional Adhesion Molecule 2/JAM-B primary antibodies -
Description
Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HUVEC, HeLa, JAR and A431 cell lysates, A431 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2489(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa. |
Target
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Function
May play a role in the processes of lymphocyte homing to secondary lymphoid organs. -
Tissue specificity
Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells. -
Sequence similarities
Belongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 58494 Human
- Entrez Gene: 67374 Mouse
- Entrez Gene: 619374 Rat
- Omim: 606870 Human
- SwissProt: P57087 Human
- SwissProt: Q9JI59 Mouse
- Unigene: 517227 Human
- Unigene: 41758 Mouse
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Alternative names
- C21orf43 antibody
- CD 322 antibody
- CD322 antibody
see all
Images
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All lanes : Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : JAR cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa -
Intracellular flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2/JAM-B using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab156586 has been referenced in 1 publication.
- Wei S et al. Development and validation of a novel fibroblast scoring model for lung adenocarcinoma. Front Oncol 12:905212 (2022). PubMed: 36072807