Product nameAnti-Junctional Adhesion Molecule 2/JAM-B antibody
See all Junctional Adhesion Molecule 2/JAM-B primary antibodies
DescriptionRabbit polyclonal to Junctional Adhesion Molecule 2/JAM-B
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Chimpanzee, Macaque monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human Junctional Adhesion Molecule 2/JAM-B aa 200 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: P57087
- This antibody gave a positive signal in Human, Mouse and Rat Heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heartmuscle.
Previously labelled as Junctional Adhesion Molecule 2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab139645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Tissue specificityHighest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- C21orf43 antibody
- CD 322 antibody
- CD322 antibody
IHC image of Junctional Adhesion Molecule 2/JAM-B staining in Human normal heartmuscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab139645, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-Junctional Adhesion Molecule 2/JAM-B antibody (ab139645) at 1 µg/ml (Milk blocking - 3%)
Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Junctional Adhesion Molecule 2/JAM-B contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
The predicted molecular weight of Junctional Adhesion Molecule 2/JAM-B is 33 kDa (SwissProt), however we expect to observe a banding pattern around 45 kDa.
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab139645 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab139645 has been referenced in 4 publications.
- Liu XQ et al. lncRNA MALAT1 Accelerates Wound Healing of Diabetic Mice Transfused with Modified Autologous Blood via the HIF-1a Signaling Pathway. Mol Ther Nucleic Acids 17:504-515 (2019). PubMed: 31344658
- Sharma UC et al. Effects of a novel peptide Ac-SDKP in radiation-induced coronary endothelial damage and resting myocardial blood flow. Cardiooncology 4:N/A (2018). PubMed: 31057947
- Hintermann E et al. Junctional adhesion molecules JAM-B and JAM-C promote autoimmune-mediated liver fibrosis in mice. J Autoimmun 91:83-96 (2018). PubMed: 29753567
- Stelzer IA et al. Differential mouse-strain specific expression of Junctional Adhesion Molecule (JAM)-B in placental structures. Cell Adh Migr 10:2-17 (2016). PubMed: 26914234