Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)]
See all Junctional Adhesion Molecule 2/JAM-B primary antibodies
DescriptionRabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
corresponding to Human Junctional Adhesion Molecule 2/JAM-B.
- HUVEC, HeLa, JAR and A431 cell lysates, A431 cells
Previously labelled as Junctional Adhesion Molecule 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab156586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 33 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Tissue specificityHighest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- C21orf43 antibody
- CD 322 antibody
- CD322 antibody
All lanes : Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : JAR cell lysates
Lane 4 : A431 cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
Flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2/JAM-B using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).
ab156586 has not yet been referenced specifically in any publications.