Recombinant
RabMAb

Recombinant Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586)

Overview

  • Product name

    Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)]
    See all Junctional Adhesion Molecule 2/JAM-B primary antibodies
  • Description

    Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human Junctional Adhesion Molecule 2/JAM-B.

  • Positive control

    • HUVEC, HeLa, JAR and A431 cell lysates, A431 cells
  • General notes

    Previously labelled as Junctional Adhesion Molecule 2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab156586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      May play a role in the processes of lymphocyte homing to secondary lymphoid organs.
    • Tissue specificity

      Highest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
    • Sequence similarities

      Belongs to the immunoglobulin superfamily.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization

      Cell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
    • Information by UniProt
    • Database links

    • Alternative names

      • C21orf43 antibody
      • CD 322 antibody
      • CD322 antibody
      • CD322 antigen antibody
      • JAM 2 antibody
      • JAM B antibody
      • JAM IT/VE JAM antibody
      • JAM, vascular endothelial antibody
      • JAM-2 antibody
      • JAM-B antibody
      • JAM2 antibody
      • JAM2_HUMAN antibody
      • JAMB antibody
      • Jcam2 antibody
      • Junction adhesion molecule 2 antibody
      • Junction adhesion molecule B antibody
      • Junctional adhesion molecule 2 antibody
      • Junctional adhesion molecule B antibody
      • PRO 245 antibody
      • PRO245 antibody
      • Vascular endothelial junction associated molecule antibody
      • Vascular endothelial junction-associated molecule antibody
      • VE JAM antibody
      • VE-JAM antibody
      • VEJAM antibody
      see all

    Images

    • All lanes : Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586) at 1/1000 dilution

      Lane 1 : HUVEC cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : JAR cell lysates
      Lane 4 : A431 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 33 kDa

    • Flow cytometric analysis of permeabilized A431 cells labeling Junctional Adhesion Molecule 2/JAM-B using ab156586 at a 1/100 dilution (red) or a rabbit IgG control (green).

    References

    ab156586 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    This product is known to not work in this application or species.
    Application
    Immunoprecipitation
    Sample
    Mouse Cell lysate - other (fibroblast)
    Total protein in input
    100 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    fibroblast

    Sir or Madam Dwscott

    Verified customer

    Submitted Jul 26 2013

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (fibroblast)
    Permeabilization
    Yes - 0.1% Triton X-100, 5 min
    Specification
    fibroblast
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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