Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] - BSA and Azide free
See all Junctional Adhesion Molecule 2/JAM-B primary antibodies
DescriptionRabbit monoclonal [EPR2489(2)] to Junctional Adhesion Molecule 2/JAM-B - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Junctional Adhesion Molecule 2/JAM-B. The exact sequence is proprietary.
Ab249274 is the carrier-free version of ab156586. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab249274 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab249274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 33 kDa.|
FunctionMay play a role in the processes of lymphocyte homing to secondary lymphoid organs.
Tissue specificityHighest expression in the heart, placenta, lung, foreskin and lymph node. Prominently expressed on high endothelial venules, also present on the endothelia of other vessels. Localized to the intercellular boundaries of high endothelial cells.
Sequence similaritiesBelongs to the immunoglobulin superfamily.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localizationCell junction > tight junction. Cell membrane. Localized at tight junctions of both epithelial and endothelial cells.
- Information by UniProt
- C21orf43 antibody
- CD 322 antibody
- CD322 antibody
All lanes : Anti-Junctional Adhesion Molecule 2/JAM-B antibody [EPR2489(2)] (ab156586) at 1/1000 dilution
Lane 1 : HUVEC cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : JAR cell lysates
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa
This data was developed using ab156586, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab249274 has not yet been referenced specifically in any publications.