Overview

  • Product name
  • Description
    Rabbit polyclonal to JunD
  • Host species
    Rabbit
  • Specificity
    ab28837 recognises JunD.
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255.

  • Positive control
    • Breast carcinoma, extracts from Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/20000.
IP Use at an assay dependent concentration.

Target

Images

  • Lane 1 : Anti-JunD antibody (ab28837) at 1/500 dilution
    Lane 2 : Anti-JunD antibody (ab28837) at 1/500 dilution (preincubated with synthesized peptide.)

    All lanes : extracts from Hela cells.

    Predicted band size: 35 kDa
    Observed band size: 43 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded breast carcinoma, using 1/50 JunD Antibody(ab28837).

References

This product has been referenced in:
  • Bigot P  et al. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun 7:12098 (2016). EMSA . Read more (PubMed: 27384883) »
  • Eagle AL  et al. Experience-Dependent Induction of Hippocampal ?FosB Controls Learning. J Neurosci 35:13773-83 (2015). Read more (PubMed: 26446228) »
See all 5 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunoprecipitation
Immuno-precipitation step
Other - EMSA
Sample
Human Cell lysate - nuclear (renal cancer cells)
Specification
renal cancer cells
Total protein in input
3 µg

Abcam user community

Verified customer

Submitted Mar 14 2014

Question
Answer

Thank you for your inquiry. Firstly, this antibody is not phospho-specific. It will recognize the total protein. The immunogen is a synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255. We got only one band at about 41kD from the WB image. According to the below reference, there are two isoforms of JunD, a full-length isoform containing 341 amino acids (JunD-FL) and a truncated isoform lacking 48 amino acids at the N terminus (ΔJunD). http://www.jbc.org/content/277/33/29710.abstract I'd imagine that this antibody could detect both isoforms due to where the immunogen falls, however we didn't perform any specific tests to see if the antibody will recognize JunD-FL or ΔJunD. I hope this information helps. Please contact us with any other questions.  

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Answer

Thank you for contacting us. The antibody was raised against a sequence around Serine 255 of human JunD. It is not a phospho antibody, though, and will bind the protein only if it is NOT phosphorylated at S255. Regarding the 2 isoforms: I checked the UniPort entry for human JunD protein (http://www.uniprot.org/uniprot/P17535) and found only one isoform listed at 35 kDa. It is the same for the mouse protein (http://www.uniprot.org/uniprot/P15066). Therefore, I am not sure how the 2 isoforms differ from each other. But as long as the sequence around S255 is present in both isoforms, the antibody will recognize both. The immunogen peptide is often between 5-15 residues long and would include S255. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. All our product are covered by Abpromise guarantee which states we replace or fully refund products not performing as stated on the datasheet when reported within 6 months of delivery. Please click the following link for full terms and conditions for Abpromise https://www.abcam.com/index.html?pageconfig=abpromise I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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