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What isoform of p-JunD this antibody can pick up? Jun D comes in 2 isoforms- 37 and 41 KDa.
Asked on Sep 13 2011
Thank you for contacting us. The antibody was raised against a sequence around Serine 255 of human JunD. It is not a phospho antibody, though, and will bind the protein only if it is NOT phosphorylated at S255. Regarding the 2 isoforms: I checked the UniPort entry for human JunD protein (http://www.uniprot.org/uniprot/P17535) and found only one isoform listed at 35 kDa. It is the same for the mouse protein (http://www.uniprot.org/uniprot/P15066). Therefore, I am not sure how the 2 isoforms differ from each other. But as long as the sequence around S255 is present in both isoforms, the antibody will recognize both. The immunogen peptide is often between 5-15 residues long and would include S255. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Sep 13 2011