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What isoform of p-JunD this antibody can pick up? Jun D comes in 2 isoforms- 37 and 41 KDa.
Asked on Sep 15 2011
Thank you for your inquiry. Firstly, this antibody is not phospho-specific. It will recognize the total protein. The immunogen is a synthetic non-phosphopeptide derived from human JunD around the phosphorylation site of Serine 255. We got only one band at about 41kD from the WB image. According to the below reference, there are two isoforms of JunD, a full-length isoform containing 341 amino acids (JunD-FL) and a truncated isoform lacking 48 amino acids at the N terminus (ΔJunD). http://www.jbc.org/content/277/33/29710.abstract I'd imagine that this antibody could detect both isoforms due to where the immunogen falls, however we didn't perform any specific tests to see if the antibody will recognize JunD-FL or ΔJunD. I hope this information helps. Please contact us with any other questions.
Answered on Sep 15 2011