Product nameAnti-JunD antibody
See all JunD primary antibodies
DescriptionRabbit polyclonal to JunD
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Chimpanzee
- This antibody gave a positive signal in the following Human Lysates: HeLa Nuclear, Jurkat Nuclear, HEK293 Whole Cell, HepG2 Nuclear
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67453 in the following tested applications.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).|
FunctionTranscription factor binding AP-1 sites.
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
All lanes : Anti-JunD antibody (ab67453) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :
Jurkat nuclear extract lysate (ab14844)
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 :
HepG2 nuclear extract lysate (ab14660)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
ICC/IF image of ab67453 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67453, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and HepG2 cells at 1µg/ml.
ab67453 has not yet been referenced specifically in any publications.