Product nameAnti-JunD antibody
See all JunD primary antibodies
DescriptionRabbit polyclonal to JunD
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Dog, Chimpanzee
Synthetic peptide corresponding to Human JunD aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following Human Nuclear Lysates: HeLa, Jurkat
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab74428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 35 kDa).|
FunctionTranscription factor binding AP-1 sites.
Sequence similaritiesBelongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
All lanes : Anti-JunD antibody (ab74428) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :
Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
The 43 kDa band observed is comparable to molecular weights seen with other commercially available antibodies to JunD.
ab74428 has not yet been referenced specifically in any publications.