Recombinant Anti-JunD antibody [EPR17365] (ab181615)

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Western blot - Anti-JunD antibody [EPR17365] (ab181615)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR17365] to JunD
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-JunD antibody [EPR17365]
See all JunD primary antibodies
Description

Rabbit monoclonal [EPR17365] to JunD
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment within Mouse JunD aa 1-150. The exact sequence is proprietary.
Database link: P15066
Positive control
- WB: 293T, Jurkat, HeLa, C6, Raw264.7, PC12, NIH 3T3 cell lysates; Human fetal liver, fetal brain, fetal heart and fetal kidney lysates. IHC-P: Human mammary gland tissue and lung squamous cell carcinoma tissue; Mouse and rat cerebral cortex tissues. ICC/IF: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR17365
Isotype

IgG
Research areas

Alternative Versions
- Anti-JunD antibody [EPR17365] - BSA and Azide free (ab250511)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human JunD protein (ab152486)

Our Abpromise guarantee covers the use of ab181615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000. Detects a band of approximately 39,42 kDa (predicted molecular weight: 35 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF		1/10000.

Function

Transcription factor binding AP-1 sites.
Sequence similarities

Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P17535 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24518 Rat
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- SwissProt: P52909 Rat
- Unigene: 2780 Human
- Unigene: 419851 Mouse
- Unigene: 46225 Rat
see all
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP1 antibody
- Jun D antibody
- jun D proto oncogene antibody
- Jund antibody
- JunD FL isoform antibody
- JUND_HUMAN antibody
- Transcription factor jun D antibody
- Transcription factor jun-D antibody
see all

Western blot - Anti-JunD antibody [EPR17365] (ab181615)

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
Lane 3 : Human fetal liver lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 39,42 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-JunD antibody [EPR17365] (ab181615)

Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 39,42 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-JunD antibody [EPR17365] (ab181615)

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 39,42 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

5% NFDM/TBST: Blocking and diluting buffer.
Western blot - Anti-JunD antibody [EPR17365] (ab181615)

All lanes : Anti-JunD antibody [EPR17365] (ab181615) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus ) whole cell lysate
Lane 3 : PC12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 4 : NIH 3T3 (Mouse embyro fibroblast cells ) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 35 kDa
Observed band size: 39,42 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

5% NFDM/TBST: Blocking and diluting buffer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note: Nuclear staining on the epithelial cells of Human mammary gland was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

Immunohistochemical analysis of paraffin-embedded Human lung squamous cell carcinoma tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note: Nucleus staining on the cancer cells of lung squamous cell carcinoma was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note: Nuclear staining on neurons of the mouse cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-JunD antibody [EPR17365] (ab181615)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling JunD using ab181615 at 1/1000 dilution. A Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as secondary at 1/500 dilution. Counterstain: Hematoxylin.
Inset image: negative control obtained using PBS instead of ab181615 and secondary antibody only.
Note: Nuclear staining on neurons of the rat cerebral cortex was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-JunD antibody [EPR17365] (ab181615)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells, labeling JunD with ab181615 at 1/10000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image shows nuclear staining on the HeLa cell line. The nuclear counter stain is DAPI (blue) . Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
1. ab181615 at 1/10000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
Anti-JunD antibody [EPR17365] (ab181615)

Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab181615? Please let us know so that we can cite the reference in this datasheet.

ab181615 has been referenced in 2 publications.

Shirali AS et al. A multi-step transcriptional cascade underlies vascular regeneration in vivo. Sci Rep 8:5430 (2018). PubMed: 29615716
Liu F et al. The role of NF-?B-mediated JNK pathway in cognitive impairment in a rat model of sleep apnea. J Thorac Dis 10:6921-6931 (2018). PubMed: 30746238

Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Recombinant Anti-JunD antibody [EPR17365] (ab181615)

Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References (2)

Customer reviews and Q&As