Overview

  • Product name

    Anti-JunD antibody [EPR6520]
    See all JunD primary antibodies
  • Description

    Rabbit monoclonal [EPR6520] to JunD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human JunD aa 100-200. The exact sequence is proprietary.

  • Positive control

    • 293T, Jurkat, HepG2 and HeLa whole cell lysate (ab150035); Human skin and kidney tissue; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 39, 43 kDa (predicted molecular weight: 35 kDa).

For unpurified, use 1/5000.

IP 1/50.

For unpurified, use 1/30.

ICC/IF 1/300.

For unpurified, use 1/200.

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Transcription factor binding AP-1 sites.
    • Sequence similarities

      Belongs to the bZIP family. Jun subfamily.
      Contains 1 bZIP domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activator protein 1 antibody
      • AP 1 antibody
      • AP1 antibody
      • Jun D antibody
      • jun D proto oncogene antibody
      • Jund antibody
      • JunD FL isoform antibody
      • JUND_HUMAN antibody
      • Transcription factor jun D antibody
      • Transcription factor jun-D antibody
      see all

    Images

    • Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 35 kDa
      Observed band size: 39,43 kDa
      why is the actual band size different from the predicted?



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 300, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, purified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • ab134067 (purified) at 1/50 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-JunD antibody [EPR6520] (ab134067) at 1/10000 dilution (unpurified)

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 39,43 kDa why is the actual band size different from the predicted?

    • Immunofluorescence staining of HeLa cells with unpurified ab134067 at a working dilution of 1 in 200, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 555 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA. The negative control is shown in bottom right hand panel - for the negative control, unpurified ab134067 was used at a dilution of 1/200 followed by an Alexa Fluor® 488 goat anti-mouse antibody at a dilution of 1/500.

    • Immunofluorescent analysis of HeLa cells labelling JunD with unpurified ab134067 at 1/100 dilution.

    • ab134067 (unpurified) at 1/30 immunoprecipitating JunD in HepG2 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Ribeiro EA  et al. Transcriptional and physiological adaptations in nucleus accumbens somatostatin interneurons that regulate behavioral responses to cocaine. Nat Commun 9:3149 (2018). Read more (PubMed: 30089879) »
    • Bigot P  et al. Functional characterization of the 12p12.1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun 7:12098 (2016). EMSA . Read more (PubMed: 27384883) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 27 2014

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Other - EMSA
    Sample
    Human Cell lysate - nuclear (Renal cancer cell lines - nuclear extract)
    Specification
    Renal cancer cell lines - nuclear extract
    Total protein in input
    3 µg

    Abcam user community

    Verified customer

    Submitted Mar 18 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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