Jurkat cytoplasmic extract lysate (ab14842)
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- References
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Overview
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Product name
Jurkat cytoplasmic extract lysate
See all Jurkat lysates -
General notes
Cell line: Jurkat (Human T cell lymphoblast-like).
We recommend aliquoting the extracts into single use fractions and then storing them. -
Tested applications
Suitable for: EMSA, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.003% EDTA, 0.24% HEPES, 0.015% DTT, 0.009% PMSF, 0.075% Potassium chloride, 0.004% EGTA, 0.0001% Leupeptin, 0.0001% Aprotinin -
Concentration information loading... -
Research areas
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Background
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Applications
Our Abpromise guarantee covers the use of ab14842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| EMSA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. |
Datasheets and documents
References
ab14842 has not yet been referenced specifically in any publications.
