Product nameJurkat cytoplasmic extract lysate
See all Jurkat lysates
General notesCell line: Jurkat (Human T cell lymphoblast-like).
We recommend aliquoting the extracts into single use fractions and then storing them.
Tested applicationsSuitable for: EMSA, WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.003% EDTA, 0.24% HEPES, 0.015% DTT, 0.009% PMSF, 0.075% Potassium chloride, 0.004% EGTA, 0.0001% Leupeptin, 0.0001% Aprotinin
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BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Our Abpromise guarantee covers the use of ab14842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
ab14842 has not yet been referenced specifically in any publications.