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  1. Link

    jurkat-cytoplasmic-extract-lysate-ab14842.pdf

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Kits/ Lysates/ Other Lysates Cytoplasmic Lysates Human Other
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Jurkat cytoplasmic extract lysate (ab14842)

  • Datasheet
Submit a review Q&A (1)

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Overview

  • Product name

    Jurkat cytoplasmic extract lysate
    See all Jurkat lysates
  • General notes

    Cell line: Jurkat (Human T cell lymphoblast-like).
    We recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applications

    Suitable for: EMSA, WBmore details

Properties

  • Mycoplasma free

    Yes
  • Form

    Liquid
  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.90
    Constituents: 0.003% EDTA, 0.24% HEPES, 0.015% DTT, 0.009% PMSF, 0.075% Potassium chloride, 0.004% EGTA, 0.0001% Leupeptin, 0.0001% Aprotinin
  • Concentration information loading...
  • Research areas

    • Kits/ Lysates/ Other
    • Lysates
    • Cytoplasmic Lysates
    • Human
    • Other
  • Background

    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab14842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.
Notes
EMSA
Use at an assay dependent dilution.
WB
Use at an assay dependent dilution.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab14842? Please let us know so that we can cite the reference in this datasheet.

ab14842 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

we are looking for a Jurkat cell line, that can be stimulated for producing detectable amounts ( ELISA/ FACS) of IL-2. Are your Jurkat cell lines tested for IL-2 production under any conditions ? We`d very thankful for a confirmation, that you recieved this email and for a fast reply ! Many thanks and Merry Christmas ! Kind regards

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Abcam community

Verified customer

Asked on Dec 23 2004

Answer

Thank you for your enquiry. We didn't assay our Jurkat cell line for IL-2 production unfortunately, I'm sorry for the inconvenience. Best wishes for 2005,

Read More

Abcam Scientific Support

Answered on Dec 30 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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