Product nameJurkat cytoplasmic extract lysate
See all Jurkat lysates
General notesCell line: Jurkat (Human T cell lymphoblast-like).
We recommend aliquoting the extracts into single use fractions and then storing them.
Tested applicationsSuitable for: EMSA, WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9
Concentration information loading...
BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Our Abpromise guarantee covers the use of ab14842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|EMSA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution.|
ab14842 has not yet been referenced specifically in any publications.