Jurkat cytoplasmic extract lysate (ab14842)
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Overview
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Product nameJurkat cytoplasmic extract lysate
See all Jurkat lysates -
General notesCell line: Jurkat (Human T cell lymphoblast-like).
We recommend aliquoting the extracts into single use fractions and then storing them. -
Tested applicationsSuitable for: EMSA, WBmore details
Properties
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FormLiquid
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Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT. pH 7.9 -
Concentration information loading... -
Research areas
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BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Applications
Our Abpromise guarantee covers the use of ab14842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| EMSA | Use at an assay dependent dilution. | |
| WB | Use at an assay dependent dilution. |
Datasheets and documents
References
ab14842 has not yet been referenced specifically in any publications.
