Jurkat cytoplasmic extract lysate (ab14842)
Overview
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Product name
Jurkat cytoplasmic extract lysate
See all Jurkat lysates -
General notes
Cell line: Jurkat (Human T cell lymphoblast-like).
We recommend aliquoting the extracts into single use fractions and then storing them. -
Tested applications
Suitable for: EMSA, WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.003% EDTA, 0.24% HEPES, 0.015% DTT, 0.009% PMSF, 0.075% Potassium chloride, 0.004% EGTA, 0.0001% Leupeptin, 0.0001% Aprotinin -
Concentration information loading... -
Research areas
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Background
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14842 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| EMSA |
Use at an assay dependent dilution.
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| WB |
Use at an assay dependent dilution.
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| Notes |
|---|
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EMSA
Use at an assay dependent dilution. |
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WB
Use at an assay dependent dilution. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab14842 has not yet been referenced specifically in any publications.
