Jurkat nuclear extract lysate (PHA stimulated) (ab14848)
Overview
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Product name
Jurkat nuclear extract lysate (PHA stimulated)
See all Jurkat lysates -
General notes
Cell line: Jurkat (Human T cell lymphoblast-like).
Cells were cultured in medium supplemented with 2 µg/ml PHA (Leucoagglutinin, PHA-L) for 2 days at 37°C immediately prior to harvesting.
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol -
Concentration information loading...
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Research areas
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Background
Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab14848 has not yet been referenced specifically in any publications.