Jurkat nuclear extract lysate (PHA stimulated) (ab14848)

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Overview

  • Product name

    Jurkat nuclear extract lysate (PHA stimulated)
    See all Jurkat lysates

  • General notes

    Cell line: Jurkat (Human T cell lymphoblast-like).
    Cells were cultured in medium supplemented with 2 µg/ml PHA (Leucoagglutinin, PHA-L) for 2 days at 37°C immediately prior to harvesting.

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.90
    Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
  • Concentration information loading...

  • Research areas

  • Background

    Often this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab14848? Please let us know so that we can cite the reference in this datasheet.

ab14848 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Question

Dear Technical Team, We have a customer here with a technical question regarding the above nuclear lysate. He wants to know which type of cytokine the Jurkat (Human T cell lymphoblast-like cell line) produces. Hope to hear from you soon. Kind regards

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Answer

Thank you for your enquiry. I can confirm that this lysate has been tested in WB for NFATc1 activation but not for the other cytokines. I am able to provide details of the conditions of induction we used to prepare this extract : Cells were cultured in medium supplemented with 2 µg/ml PHA (Leucoagglutinin, PHA-L) for 2 days at 37°C immediately prior to harvesting. I hope this is helpful. If you have any further questions, please do not hesitate to contact us.

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