Product nameJurkat nuclear extract lysate (TPA + CI stimulated)
See all Jurkat lysates
General notesCell line: Jurkat (Human T cell lymphoblast-like).
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 µM calcium ionophore A23187 (CI) for two hours at 37°C immediately prior to harvesting.
Storage instructionsAliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.90
Constituents: 0.75% Potassium chloride, 0.009% PMSF, 0.01% Magnesium chloride, 0.008% DTT, 0.48% HEPES, 20% Glycerol
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BackgroundOften this cell line is called 'JM' (Jurkat and JM are derived from the same patient and are sister clones), occasionally JM may be a subclone with somewhat divergent features. Jurkat cells can be transfected and are therefore useful for studies of blood proto-oncogenes expression, apoptosis and cell survival (e.g HIV). They produce IL-2 and can also be used for studies of differentiation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab14850 has not yet been referenced specifically in any publications.