K-562 whole cell lysate (ab7911)

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Overview

  • Product name

    K-562 whole cell lysate
    See all K562 lysates

  • Description

    K-562 cell lysate

  • General notes

    Cell line: K562 (Human Chronic Myelogenous Leukemia).
    Growth media: RPMI and 10% NCS (Newborn calf serum).

     

    K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • Tested applications

    Suitable for: WBmore details

Properties

  • Mycoplasma free

    Yes

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Constituents: 0.77% DTT, 1% Sodium Lauryl Sulfate, 12.5% Glycerol (glycerin, glycerine), 0.01% Bromophenol blue, 0.79% Tris HCl
  • Concentration information loading...

  • Lysate notes

    K562 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50mM DTT.

  • Research areas

  • Background

    K-562 is an erythroleukemia cell line derived from a chronic myeloid leukemia patient in blast crisis. Recent studies have shown the K562 blasts are multipotential, hematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series. Cells are also used for haemoglobin synthesis, antitumour testing, tumorigenicity, differentiation, pharmacodynamics, cloning, cytotoxicity, cell biology, cellular effects of hyperthermia, and natural killer assays.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration.

K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
Notes
WB
Use at an assay dependent concentration.

K562 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (1)

Publishing research using ab7911? Please let us know so that we can cite the reference in this datasheet.

ab7911 has been referenced in 1 publication.

  • Baglo Y  et al. Enhanced efficacy of bleomycin in bladder cancer cells by photochemical internalization. Biomed Res Int 2014:921296 (2014). WB . PubMed: 25101299

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

We are currently doing western blot analysis using "Anti-FNDC5 antibody-Carboxyterminal end (ab93373)" from abcam. As its datasheet mentions, K562 cell line lysate was used as positive control, which we are planning to orderas soon as possible. However, we are not sure whether we should order "K562 whole cell lysate-tumor cell line (ab29306)" or "k562 whole cell lysate (ab7911)" for this purpose. Moreover, could you please kindly email me the western blot condition when using this lysate?

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Answer

Thank you for contacting us.

The lysates ab29306 and ab7911 were prepared using slightly different techniques, but will give equivalent results in WB. Either of these lysates will be guaranteed for use as a positive control with ab93373. We recommendloading 20ug of lysate per lane. Ab29306should be boiled for 5 minutes prior to loading in thegel, but ab7911 has already beenboiled.I hope this helps, please let me know if you need any additional information or assistance.

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Question

What is the cell concentration (cells/mL) of the lysate?

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Answer

Thanks for your call yesterday and for your patience while I have been in touch with the lab regarding your enquiry. The lysate is vialed in terms of protein concentration, so unfortunately the lab does not have any information about the cell concentration. I am sorry we don't have the information you are looking for, but if there is anything else that we can help you with please let me know.

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