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We are currently doing western blot analysis using "Anti-FNDC5 antibody-Carboxyterminal end (ab93373)" from abcam. As its datasheet mentions, K562 cell line lysate was used as positive control, which we are planning to orderas soon as possible. However, we are not sure whether we should order "K562 whole cell lysate-tumor cell line (ab29306)" or "k562 whole cell lysate (ab7911)" for this purpose. Moreover, could you please kindly email me the western blot condition when using this lysate?
Asked on Jun 18 2012
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The lysates ab29306 and ab7911 were prepared using slightly different techniques, but will give equivalent results in WB. Either of these lysates will be guaranteed for use as a positive control with ab93373. We recommendloading 20ug of lysate per lane. Ab29306should be boiled for 5 minutes prior to loading in thegel, but ab7911 has already beenboiled.I hope this helps, please let me know if you need any additional information or assistance.
Answered on Jun 18 2012