Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10943] to K23
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-K23 antibody [EPR10943]
See all K23 primary antibodies
DescriptionRabbit monoclonal [EPR10943] to K23
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human K23.
Database link: Q9C075
- HepG2, JAR, and BxPC-3 cell lysates, Human gastric carcinoma tissue, JAR cells
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab156569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK 23 antibody
- CK-23 antibody
- CK23 antibody
All lanes : Anti-K23 antibody [EPR10943] (ab156569) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : JAR cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling K23 with ab156569 antibody at 1/150.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometrical analysis of permeabilized JAR cells with ab156569 antibody at a dilution of 1/10 (red) compared to a rabbit IgG (green).
ab156569 has been referenced in 1 publication.
- Kinast V et al. Identification of Keratin 23 as a Hepatitis C Virus-Induced Host Factor in the Human Liver. Cells 8:N/A (2019). PubMed: 31216713