Recombinant Anti-K23 antibody [EPR10943] - BSA and Azide free (ab249264)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10943] to K23 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-K23 antibody [EPR10943] - BSA and Azide free
See all K23 primary antibodies -
Description
Rabbit monoclonal [EPR10943] to K23 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human K23.
Database link: Q9C075 -
General notes
ab249264 is the carrier-free version of ab156569.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10943 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249264 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Sequence similarities
Belongs to the intermediate filament family. - Information by UniProt
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Database links
- Entrez Gene: 25984 Human
- Omim: 606194 Human
- SwissProt: Q9C075 Human
- Unigene: 9029 Human
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Alternative names
- CK 23 antibody
- CK-23 antibody
- CK23 antibody
see all
Images
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All lanes : Anti-K23 antibody [EPR10943] (ab156569) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : JAR cell lysate
Lane 3 : BxPC-3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDaThis data was developed using ab156569, the same antibody clone in a different buffer formulation.
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This data was developed using ab156569, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling K23 with ab156569 antibody at 1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249264 has not yet been referenced specifically in any publications.