Overview

  • Product name

    Anti-K27 antibody
  • Description

    Rabbit polyclonal to K27
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human K27 aa 10-46.
    Sequence:

    RRLGSCGGTGSVRLSSGGAGFGAGNTCGVPGIGSGFS


    Database link: Q7Z3Y8

  • Positive control

    • IHC-P - Human hair tissue.
  • General notes

    Previously labelled as KRT27. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab220725 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2500 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    K27 is a member of the type I (acidic) keratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. The type I keratin genes are clustered in a region of chromosome 17q12-q21.
  • Cellular localization

    Cytoplasm; intermediate filament.
  • Database links

  • Alternative names

    • CK 27 antibody
    • CK27 antibody
    • Cytokeratin 27 antibody
    • K25C antibody
    • K27 antibody
    • Keratin 25C antibody
    • Keratin 27 antibody
    • Keratin type I cytoskeletal 27 antibody
    • Keratin27 antibody
    • KRT 25C antibody
    • KRT 27 antibody
    • KRT25C antibody
    • KRT27 antibody
    • Type I inner root sheath specific keratin 25 irs3 antibody
    • Type I inner root sheath specific keratin K25irs3 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human hair tissue labeling K27 with ab220725 at 1/2500 dilution.

References

ab220725 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab220725.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up