Product nameAnti-K31 antibody [EPR12855(B)]
See all K31 primary antibodies
DescriptionRabbit monoclonal [EPR12855(B)] to K31
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide within Human K31 aa 300-400 (Cysteine residue). The exact sequence is proprietary.
Database link: Q15323
- Human skin lysate; Human skin tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab177955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
Tissue specificityPresent in scalp but not in hairless skin. Abundantly expressed in the differentiating cortex of growing (anagen) hair. Expression is restricted to the keratinocytes of the hair cortex and is absent from inner root sheath and medulla.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- Ha-1 antibody
- HA1 antibody
- Hair Keratin acidic hHa1 antibody
Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling K31 with ab177955 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-K31 antibody [EPR12855(B)] (ab177955) at 1/1000 dilution + Human skin lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 47 kDa
ab177955 has not yet been referenced specifically in any publications.