Recombinant
RabMAb

Recombinant Anti-K73 antibody [EPR12564-39] (ab181383)

Overview

  • Product name

    Anti-K73 antibody [EPR12564-39]
    See all K73 primary antibodies
  • Description

    Rabbit monoclonal [EPR12564-39] to K73
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human K73. The exact sequence is proprietary.
    Database link: Q86Y46

  • Positive control

    • Human skin, rat skin and mouse skin lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12564-39
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab181383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Predicted molecular weight: 59 kDa.

Target

  • Function

    Has a role in hair formation. Specific component of keratin intermediate filaments in the inner root sheath (IRS) of the hair follicle.
  • Tissue specificity

    Highly expressed in hair follicles from scalp. In hair, it is specifically present in the inner root sheath (IRS) of the hair follicle. Present in the IRS cuticle, but not in Henle or Huxley layers of the IRS. In the IRS cuticle, it is expressed between the lowermost bulb region of the cuticle and the region where Henle cells undergo abrupt terminal differentiation. Detected up to the uppermost cortex region where cuticle cells terminally differentiate (at protein level).
  • Sequence similarities

    Belongs to the intermediate filament family.
  • Information by UniProt
  • Database links

  • Alternative names

    • CK-73 antibody
    • Cytokeratin-73 antibody
    • IRT6IRS3 antibody
    • K2C73_HUMAN antibody
    • K6IRS3 antibody
    • K73 antibody
    • Keratin 6 irs3 antibody
    • Keratin, type II cytoskeletal 73 antibody
    • Keratin-73 antibody
    • KRT6IRS3 antibody
    • Krt73 antibody
    • Type II inner root sheath-specific keratin-K6irs3 antibody
    • Type-II keratin Kb36 antibody
    see all

Images

  • All lanes : Anti-K73 antibody [EPR12564-39] (ab181383) at 1/10000 dilution

    Lane 1 : Human skin lysate
    Lane 2 : Rat skin lysate
    Lane 3 : Mouse skin lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa

References

ab181383 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181383.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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