Key features and details
- Rabbit polyclonal to K80
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-K80 antibody
See all K80 primary antibodies
DescriptionRabbit polyclonal to K80
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB Control siRNA transfected MCF7 whole cell lysate; ICC: A431 whole cells.
Previously labelled as Keratin 80.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab222325 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
Tissue specificityWeakly expressed in tongue, but not skin or in any other tissues or organs examined.
Sequence similaritiesBelongs to the intermediate filament family.
- Information by UniProt
- CK 80 antibody
- CK-80 antibody
- CK80 antibody
All lanes : Anti-K80 antibody (ab222325) at 0.4 µg/ml
Lane 1 : Control siRNA transfected - MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : siRNA probe #1 transfected - MCF7 whole cell lysate
Lane 3 : siRNA probe #2 transfected - MCF7 whole cell lysate
Loading control: Anti-GAPDH.
Immunocytochemical analysis of A431 (Human epidermoid carcinoma cell line) whole cells labeling K80 (green) in the intermediate filaments with ab222325 at 2 µg/ml.
ab222325 has not yet been referenced specifically in any publications.