Anti-KA1 antibody (ab67404)
Key features and details
- Rabbit polyclonal to KA1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
-
Product name
Anti-KA1 antibody
See all KA1 primary antibodies -
Description
Rabbit polyclonal to KA1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
A 14 amino acid peptide near the amino terminus of human KA1.
-
Positive control
- Rat brain lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa).
|
Notes |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa). |
Target
-
Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 24406 Rat
- SwissProt: Q01812 Rat
- Unigene: 10049 Rat
-
Alternative names
- EAA 1 antibody
- EAA1 antibody
- Excitatory amino acid receptor 1 antibody
see all
Images
-
Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
All lanes : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
Observed band size: <97 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa. We are unsure as to the identity of these extra bands.
N.B. Despite its predicted molecular weight, KA1 often migrates at a lower molecular weight in SDS-PAGE.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (3)
ab67404 has been referenced in 3 publications.
- Ciarpella F et al. Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity. iScience 24:103438 (2021). PubMed: 34901791
- Gu L et al. Erythropoietin exerts a neuroprotective function against glutamate neurotoxicity in experimental diabetic retina. Invest Ophthalmol Vis Sci 55:8208-22 (2014). PubMed: 25335981
- Marrocco J et al. Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus. J Neurosci 32:17143-54 (2012). Rat . PubMed: 23197707