Key features and details
- Rabbit polyclonal to KA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KA1 antibody
See all KA1 primary antibodies
DescriptionRabbit polyclonal to KA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
A 14 amino acid peptide near the amino terminus of human KA1.
- Rat brain lysate and human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab67404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately <97 kDa (predicted molecular weight: 107 kDa).|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionReceptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.
Sequence similaritiesBelongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIK4 subfamily.
Cellular localizationCell membrane. Cell junction > synapse > postsynaptic cell membrane.
- Information by UniProt
- EAA 1 antibody
- EAA1 antibody
- Excitatory amino acid receptor 1 antibody
Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
All lanes : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
Observed band size: <97 kDa why is the actual band size different from the predicted?
Additional bands at: 108 kDa. We are unsure as to the identity of these extra bands.
N.B. Despite its predicted molecular weight, KA1 often migrates at a lower molecular weight in SDS-PAGE.
ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P.
ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunofluorescence of Grik4 in Human Brain cells using ab67404 at 20 ug/ml.
ab67404 has been referenced in 2 publications.
- Gu L et al. Erythropoietin exerts a neuroprotective function against glutamate neurotoxicity in experimental diabetic retina. Invest Ophthalmol Vis Sci 55:8208-22 (2014). PubMed: 25335981
- Marrocco J et al. Anxiety-like behavior of prenatally stressed rats is associated with a selective reduction of glutamate release in the ventral hippocampus. J Neurosci 32:17143-54 (2012). Rat . PubMed: 23197707