Key features and details
- Rabbit polyclonal to KACL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KACL antibody
DescriptionRabbit polyclonal to KACL
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human KACL aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Human Spleen Tissue; Human Thymus Tissue; Human Small Intestine Tissue; Human Bone Marrow Tissue; Raji Whole Cell; Jurkat Whole Cell; MOLT4 Whole Cell.
Previously labelled as CLEC2A.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 20 kDa).|
FunctionPlays a role in modulating the extent of T-cell expansion. Enhances the expansion of TCR-stimulated T cells by increasing their survival through enhanced expression of anti-apoptotic proteins. May modulate the capacity of T-cells to home to lymph nodes through SELL. Facilitates dedicated immune recognition of keratinocytes via interaction with its receptor KLRF2 by stimulating natural killer cell mediated cytotoxicity.
Tissue specificityMainly expressed in skin. Also expressed in keratinocytes, spleen, thymus, small intestine, peripheral blood monocytes, bone marrow, ovary, testis and skin. High expression in CD8(+), B lymphocytes and naive CD4(+) T cells. Restricted mostly to proliferating lymphocytes. Not detected in myeloid leukocytes or natural killer (NK) cells.
Sequence similaritiesContains 1 C-type lectin domain.
Cellular localizationCell membrane.
- Information by UniProt
- C-type lectin domain family 2 member A antibody
- CLC2A_HUMAN antibody
- CLEC2A antibody
All lanes : Anti-KACL antibody (ab76711) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human thymus tissue lysate - total protein (ab30146)
Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 7 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Additional bands at: 300 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
KACL contains a potential glycosylation site (SwissProt), which may explain its migration at a higher molecular weight than predicted. The band at 32-kDa is consistent with what has been reported in the literature (PMID:16174766).
ab76711 has not yet been referenced specifically in any publications.