Recombinant Anti-Kaiso antibody [EPR4668] (ab124777)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4668] to Kaiso
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Kaiso antibody [EPR4668]
See all Kaiso primary antibodies -
Description
Rabbit monoclonal [EPR4668] to Kaiso -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Kaiso aa 600-700. The exact sequence is proprietary.
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Positive control
- WB: Human heart, MCF7, C6, PC12 or NIH 3T3 lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4668 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124777 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 74 kDa). |
Target
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Function
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. -
Tissue specificity
Expressed in vascular endothelium. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 3 C2H2-type zinc fingers. -
Cellular localization
Nucleus. Cytoplasm. Also cytoplasmic in cells grown at high densities. - Information by UniProt
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Database links
- Entrez Gene: 10009 Human
- Entrez Gene: 56805 Mouse
- Entrez Gene: 501506 Rat
- Omim: 300329 Human
- SwissProt: Q86T24 Human
- SwissProt: Q8BN78 Mouse
- Unigene: 143604 Human
- Unigene: 391248 Mouse
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Alternative names
- Kaiso antibody
- Kaiso protein antibody
- Kaiso transcription factor antibody
see all
Images
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All lanes : Anti-Kaiso antibody [EPR4668] (ab124777) at 1/1000 dilution
Lane 1 : Human heart lysate
Lane 2 : MCF7 lysate
Lane 3 : C6 lysate
Lane 4 : PC12 lysate
Lane 5 : NIH 3T3 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 74 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab124777 has been referenced in 2 publications.
- Chen H et al. Overexpression of miR-1283 Inhibits Cell Proliferation and Invasion of Glioma Cells by Targeting ATF4. Oncol Res 27:325-334 (2019). PubMed: 29716673
- Wang L et al. Kaiso (ZBTB33) Downregulation by Mirna-181a Inhibits Cell Proliferation, Invasion, and the Epithelial-Mesenchymal Transition in Glioma Cells. Cell Physiol Biochem 48:947-958 (2018). PubMed: 30036882