Product nameAnti-Kallikrein 14 antibody [EPR7209]
See all Kallikrein 14 primary antibodies
DescriptionRabbit monoclonal [EPR7209] to Kallikrein 14
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Kallikrein 14 aa 250-350 (C terminal). The exact sequence is proprietary.
- SH-SY5Y, fetal liver, fetal kidney, HACAT and prostate cancer lysates
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.51 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab128957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 29, 31 kDa (predicted molecular weight: 29 kDa).|
FunctionSerine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.
Tissue specificityHighly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level).
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
modificationsProteolytic cleavage of the activation peptide produces the active enzyme.
Cellular localizationSecreted > extracellular space.
- Information by UniProt
- hK14 antibody
- Kallikrein 14 antibody
- Kallikrein like protein 6 antibody
All lanes : Anti-Kallikrein 14 antibody [EPR7209] (ab128957) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : Fetal kidney lysate
Lane 4 : HACAT cell lysate
Lane 5 : Prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
Observed band size: 29,31 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab128957 has been referenced in 1 publication.
- Williams MR et al. Staphylococcus aureus Induces Increased Serine Protease Activity in Keratinocytes. J Invest Dermatol 137:377-384 (2017). PubMed: 27765722